Mechanosensitive Hair Cell-like Cells from Embryonic and Induced Pluripotent Stem Cells

Kazuo Oshima, Kunyoo Shin, Marc Diensthuber, Anthony W. Peng, Anthony J. Ricci, Stefan Heller

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

Mechanosensitive sensory hair cells are the linchpin of our senses of hearing and balance. The inability of the mammalian inner ear to regenerate lost hair cells is the major reason for the permanence of hearing loss and certain balance disorders. Here, we present a stepwise guidance protocol starting with mouse embryonic stem and induced pluripotent stem cells, which were directed toward becoming ectoderm capable of responding to otic-inducing growth factors. The resulting otic progenitor cells were subjected to varying differentiation conditions, one of which promoted the organization of the cells into epithelial clusters displaying hair cell-like cells with stereociliary bundles. Bundle-bearing cells in these clusters responded to mechanical stimulation with currents that were reminiscent of immature hair cell transduction currents.

Original languageEnglish (US)
Pages (from-to)704-716
Number of pages13
JournalCell
Volume141
Issue number4
DOIs
StatePublished - May 2010
Externally publishedYes

Fingerprint

Induced Pluripotent Stem Cells
Stem cells
Cells
Audition
Bearings (structural)
Ear
Intercellular Signaling Peptides and Proteins
Ectoderm
Inner Ear
Hearing Loss
Hearing
Stem Cells
Epithelial Cells

Keywords

  • Humdisease
  • Molneuro
  • Stemcell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Oshima, K., Shin, K., Diensthuber, M., Peng, A. W., Ricci, A. J., & Heller, S. (2010). Mechanosensitive Hair Cell-like Cells from Embryonic and Induced Pluripotent Stem Cells. Cell, 141(4), 704-716. https://doi.org/10.1016/j.cell.2010.03.035

Mechanosensitive Hair Cell-like Cells from Embryonic and Induced Pluripotent Stem Cells. / Oshima, Kazuo; Shin, Kunyoo; Diensthuber, Marc; Peng, Anthony W.; Ricci, Anthony J.; Heller, Stefan.

In: Cell, Vol. 141, No. 4, 05.2010, p. 704-716.

Research output: Contribution to journalArticle

Oshima, K, Shin, K, Diensthuber, M, Peng, AW, Ricci, AJ & Heller, S 2010, 'Mechanosensitive Hair Cell-like Cells from Embryonic and Induced Pluripotent Stem Cells', Cell, vol. 141, no. 4, pp. 704-716. https://doi.org/10.1016/j.cell.2010.03.035
Oshima K, Shin K, Diensthuber M, Peng AW, Ricci AJ, Heller S. Mechanosensitive Hair Cell-like Cells from Embryonic and Induced Pluripotent Stem Cells. Cell. 2010 May;141(4):704-716. https://doi.org/10.1016/j.cell.2010.03.035
Oshima, Kazuo ; Shin, Kunyoo ; Diensthuber, Marc ; Peng, Anthony W. ; Ricci, Anthony J. ; Heller, Stefan. / Mechanosensitive Hair Cell-like Cells from Embryonic and Induced Pluripotent Stem Cells. In: Cell. 2010 ; Vol. 141, No. 4. pp. 704-716.
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