@article{dabff68e2d4742cda61eb53ee78cd273,
title = "Mechanosensitive Hair Cell-like Cells from Embryonic and Induced Pluripotent Stem Cells",
abstract = "Mechanosensitive sensory hair cells are the linchpin of our senses of hearing and balance. The inability of the mammalian inner ear to regenerate lost hair cells is the major reason for the permanence of hearing loss and certain balance disorders. Here, we present a stepwise guidance protocol starting with mouse embryonic stem and induced pluripotent stem cells, which were directed toward becoming ectoderm capable of responding to otic-inducing growth factors. The resulting otic progenitor cells were subjected to varying differentiation conditions, one of which promoted the organization of the cells into epithelial clusters displaying hair cell-like cells with stereociliary bundles. Bundle-bearing cells in these clusters responded to mechanical stimulation with currents that were reminiscent of immature hair cell transduction currents.",
keywords = "Humdisease, Molneuro, Stemcell",
author = "Kazuo Oshima and Kunyoo Shin and Marc Diensthuber and Peng, {Anthony W.} and Ricci, {Anthony J.} and Stefan Heller",
note = "Funding Information: We thank H. Zeng (Stanford Transgenic Research Center) for help with ESC line derivation; J. Johnson (UT Southwestern) for Math1/nGFP mice; U. Mueller (Scripps) for cadherin 23 antibody; G. Richardson for hair cell antigen antibody; F. Salles (National Institute on Deafness and Other Communication Disorders) for advice in electron microscopy sample preparation; L. Joubert (Stanford EM core facility) for expert assistance; M. Yazawa, R. Dolmetsch, and A. Ootani for help with teratoma assays; and K. Masaki, V. Starlinger, E. Corrales, M. Herget, Z. Guo, T. Jan, and S. Sinkkonen for fruitful discussions; M.D. is a fellow of the Alexander-von-Humboldt Foundation. This work was supported by National Institutes of Health grants DC006167 and P30DC010363, by the California Institute for Regenerative Medicine (grant RC1-00119-1), and by a Neuroscience of Brain Disorders Award from the McKnight Endowment Fund for Neuroscience. ",
year = "2010",
month = may,
doi = "10.1016/j.cell.2010.03.035",
language = "English (US)",
volume = "141",
pages = "704--716",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",
}