Mechanistic comparisons among base excision repair glycosylases

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The mechanisms by which various DNA glycosylases initiate the base excision repair pathways are discussed. Fundamental distinctions are made between "simple glycosylases," that do not form DNA single-strand breaks, and "glycosylases/abasic site lyases," that do form single-strand breaks. Several groupings of BER substrate sites are defined and some interactions between these groupings and glycosylase mechanisms discussed. Two characteristics are proposed to be common among all BER glycosylases: a nucleotide flipping step that serves to expose the scissile glycosyl bond to catalysis, and a glycosylase transition state characterized by substantial tetrahedral character at the base glycosyl atom.

Original languageEnglish (US)
Pages (from-to)678-682
Number of pages5
JournalFree Radical Biology and Medicine
Volume32
Issue number8
DOIs
StatePublished - Apr 15 2002
Externally publishedYes

Fingerprint

DNA Glycosylases
Single-Stranded DNA Breaks
Lyases
Catalysis
DNA Repair
Repair
Nucleotides
Atoms
DNA
Substrates

Keywords

  • Base flipping
  • DNA glycosylases
  • Free radical
  • Nucleotide flipping

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Mechanistic comparisons among base excision repair glycosylases. / Dodson, M. L.; Lloyd, Robert (Stephen).

In: Free Radical Biology and Medicine, Vol. 32, No. 8, 15.04.2002, p. 678-682.

Research output: Contribution to journalArticle

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