Mechanisms of parturition: The transfer of prostaglandin E2 and 5-hydroxyeicosatetraenoic acid across fetal membranes

Phillip R. Bennett, Geoffrey V P Chamberlain, Lata Patel, Murdoch G. Elder, Leslie Myatt

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Prostaglandin E2 production by amnion is thought to be an important event in the onset of human labor. It has been suggested that 5-hydroxyeicosatetraenoic acid, also produced in the amnion, may mediate prelabor contractions. For either of these compounds to play a paracrine role they need to cross the chorion, which has a high capacity to metabolize prostaglandins. With the use of an in vitro system we have shown that both prostaglandin E2 and 5-hydroxyeicosatetraenoic acid cross either amnion or intact amnion-chorion-decidua at a rate similar to that of an extracellular marker sucrose. Analysis by high performance liquid chromatography revealed that at physiologic concentrations neither prostaglandin E2 nor 5-hydroxyeicosatetraenoic acid were metabolized by amnion alone. Moreover, 100% of the 5-hydroxyeicosatetraenoic acid and 72% of the prostaglandin E2 remained in the active form after passage across intact amnion-chorion-decidua. There did not appear to be any difference in the rate of transfer or permiability of the membranes before or after spontaneous labor. We conclude that both 5-hydroxyeicosatetraenoic acid and prostaglandin E2 synthesised in the amnion can cross the membranes by diffusion through the extracellular space, remaining largely unmetabolized, and may play a role in the onset of human labor.

Original languageEnglish (US)
Pages (from-to)683-687
Number of pages5
JournalAmerican Journal of Obstetrics and Gynecology
Volume162
Issue number3
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Extraembryonic Membranes
Amnion
Dinoprostone
Parturition
Chorion
Labor Onset
Decidua
Membranes
Extracellular Space
5-hydroxy-6,8,11,14-eicosatetraenoic acid
Prostaglandins
Sucrose
High Pressure Liquid Chromatography

Keywords

  • 5-hydroxyeicosatetraenoic acid
  • amnion
  • chorion
  • Labor
  • parturition
  • prostaglandin E

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Mechanisms of parturition : The transfer of prostaglandin E2 and 5-hydroxyeicosatetraenoic acid across fetal membranes. / Bennett, Phillip R.; Chamberlain, Geoffrey V P; Patel, Lata; Elder, Murdoch G.; Myatt, Leslie.

In: American Journal of Obstetrics and Gynecology, Vol. 162, No. 3, 1990, p. 683-687.

Research output: Contribution to journalArticle

Bennett, Phillip R. ; Chamberlain, Geoffrey V P ; Patel, Lata ; Elder, Murdoch G. ; Myatt, Leslie. / Mechanisms of parturition : The transfer of prostaglandin E2 and 5-hydroxyeicosatetraenoic acid across fetal membranes. In: American Journal of Obstetrics and Gynecology. 1990 ; Vol. 162, No. 3. pp. 683-687.
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