Mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome (hemopoietic dysplasia)

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    Abstract

    We have analyzed mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome by culturing in agar and methylcellulose bone marrow cells from 5 glucocorticosteroid-responsive patients with the preleukemic syndrome. We have found that methylprednisolone and cortisol are equally effective in enhancing colony growth in these patients, that enhancement occurs in either methylcellulose or agar matrix and in RPMI 1640, McCoy's 5a, and alpha medium. In 4 patients, glucocorticosteroids inhibited the activity of T-lymphocyte inhibitors of granulopoiesis in vitro. In one patient, these agents stimulated the immediate proliferation of CFU-C and in the presence of LCM enhanced CFU-C survival in suspension culture. These effects were blocked by progesterone and not due to enhancement of CSA production or inhibition of inhibitory T-cell function. Our results document the involvement of cortisol sensitive T-lymphocytes in the suppression of granulopoiesis in a small number of patients with the preleukemic syndrome and indicate that the mechanisms of glucocorticosteroid responses among patients with this syndrome are heterogeneous.

    Original languageEnglish (US)
    Pages (from-to)571-580
    Number of pages10
    JournalLeukemia Research
    Volume4
    Issue number6
    DOIs
    StatePublished - 1980

    Fingerprint

    Methylcellulose
    T-Lymphocytes
    Agar
    Hydrocortisone
    Methylprednisolone
    Bone Marrow Cells
    Progesterone
    Suspensions
    Survival
    Growth
    McCoy's 5A medium
    In Vitro Techniques

    Keywords

    • CFU-C
    • Glucocorticosteroids
    • preleukemic syndrome
    • T-lymphocytes

    ASJC Scopus subject areas

    • Cancer Research
    • Hematology
    • Oncology

    Cite this

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    title = "Mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome (hemopoietic dysplasia)",
    abstract = "We have analyzed mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome by culturing in agar and methylcellulose bone marrow cells from 5 glucocorticosteroid-responsive patients with the preleukemic syndrome. We have found that methylprednisolone and cortisol are equally effective in enhancing colony growth in these patients, that enhancement occurs in either methylcellulose or agar matrix and in RPMI 1640, McCoy's 5a, and alpha medium. In 4 patients, glucocorticosteroids inhibited the activity of T-lymphocyte inhibitors of granulopoiesis in vitro. In one patient, these agents stimulated the immediate proliferation of CFU-C and in the presence of LCM enhanced CFU-C survival in suspension culture. These effects were blocked by progesterone and not due to enhancement of CSA production or inhibition of inhibitory T-cell function. Our results document the involvement of cortisol sensitive T-lymphocytes in the suppression of granulopoiesis in a small number of patients with the preleukemic syndrome and indicate that the mechanisms of glucocorticosteroid responses among patients with this syndrome are heterogeneous.",
    keywords = "CFU-C, Glucocorticosteroids, preleukemic syndrome, T-lymphocytes",
    author = "Bagby, {Grover C.}",
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    T1 - Mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome (hemopoietic dysplasia)

    AU - Bagby, Grover C.

    PY - 1980

    Y1 - 1980

    N2 - We have analyzed mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome by culturing in agar and methylcellulose bone marrow cells from 5 glucocorticosteroid-responsive patients with the preleukemic syndrome. We have found that methylprednisolone and cortisol are equally effective in enhancing colony growth in these patients, that enhancement occurs in either methylcellulose or agar matrix and in RPMI 1640, McCoy's 5a, and alpha medium. In 4 patients, glucocorticosteroids inhibited the activity of T-lymphocyte inhibitors of granulopoiesis in vitro. In one patient, these agents stimulated the immediate proliferation of CFU-C and in the presence of LCM enhanced CFU-C survival in suspension culture. These effects were blocked by progesterone and not due to enhancement of CSA production or inhibition of inhibitory T-cell function. Our results document the involvement of cortisol sensitive T-lymphocytes in the suppression of granulopoiesis in a small number of patients with the preleukemic syndrome and indicate that the mechanisms of glucocorticosteroid responses among patients with this syndrome are heterogeneous.

    AB - We have analyzed mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome by culturing in agar and methylcellulose bone marrow cells from 5 glucocorticosteroid-responsive patients with the preleukemic syndrome. We have found that methylprednisolone and cortisol are equally effective in enhancing colony growth in these patients, that enhancement occurs in either methylcellulose or agar matrix and in RPMI 1640, McCoy's 5a, and alpha medium. In 4 patients, glucocorticosteroids inhibited the activity of T-lymphocyte inhibitors of granulopoiesis in vitro. In one patient, these agents stimulated the immediate proliferation of CFU-C and in the presence of LCM enhanced CFU-C survival in suspension culture. These effects were blocked by progesterone and not due to enhancement of CSA production or inhibition of inhibitory T-cell function. Our results document the involvement of cortisol sensitive T-lymphocytes in the suppression of granulopoiesis in a small number of patients with the preleukemic syndrome and indicate that the mechanisms of glucocorticosteroid responses among patients with this syndrome are heterogeneous.

    KW - CFU-C

    KW - Glucocorticosteroids

    KW - preleukemic syndrome

    KW - T-lymphocytes

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