Measurements of astrocyte proliferation

Marina Guizzetti, Terrance J. Kavanagh, Lucio G. Costa

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Astrocytes, the most abundant cell type in the brain, proliferate during brain development. While it is generally accepted that mature astrocytes do not proliferate, neural stem cells, which have characteristics of astrocytes, retain the ability of self-renewal. Furthermore, astrocytes can regain their proliferative properties under pathological situations, such as reactive astrogliosis, a consequence of brain injury and brain tumors. Measurements of astrocyte proliferation can thus be used in investigations of physiological and pathological processes occurring in the developing and the adult brain. This chapter describes two methods for the determination of astrocyte proliferation: the incorporation of a radioactive nucleotide [3H]thymidine into DNA, which occurs during the process of DNA synthesis preceding cell division, and the flow cytometric analysis of cell cycle progression through the different phases of the cell cycle by BrDu/Hoechst and ethidium bromide labeling.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages349-359
Number of pages11
Volume758
ISBN (Print)9781617791697
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume758
ISSN (Print)10643745

Fingerprint

Astrocytes
Cell Cycle
Brain
Physiological Phenomena
Ethidium
Neural Stem Cells
DNA
Pathologic Processes
Bromodeoxyuridine
Brain Neoplasms
Cell Division
Thymidine
Brain Injuries
Nucleotides

Keywords

  • Astrocytes
  • Bromodeoxyuridine
  • Cell cycle progression
  • Cell proliferation
  • DNA synthesis
  • Flow cytometer
  • Hoechst

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Guizzetti, M., Kavanagh, T. J., & Costa, L. G. (2011). Measurements of astrocyte proliferation. In Methods in Molecular Biology (Vol. 758, pp. 349-359). (Methods in Molecular Biology; Vol. 758). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-170-3_24

Measurements of astrocyte proliferation. / Guizzetti, Marina; Kavanagh, Terrance J.; Costa, Lucio G.

Methods in Molecular Biology. Vol. 758 Humana Press Inc., 2011. p. 349-359 (Methods in Molecular Biology; Vol. 758).

Research output: Chapter in Book/Report/Conference proceedingChapter

Guizzetti, M, Kavanagh, TJ & Costa, LG 2011, Measurements of astrocyte proliferation. in Methods in Molecular Biology. vol. 758, Methods in Molecular Biology, vol. 758, Humana Press Inc., pp. 349-359. https://doi.org/10.1007/978-1-61779-170-3_24
Guizzetti M, Kavanagh TJ, Costa LG. Measurements of astrocyte proliferation. In Methods in Molecular Biology. Vol. 758. Humana Press Inc. 2011. p. 349-359. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-170-3_24
Guizzetti, Marina ; Kavanagh, Terrance J. ; Costa, Lucio G. / Measurements of astrocyte proliferation. Methods in Molecular Biology. Vol. 758 Humana Press Inc., 2011. pp. 349-359 (Methods in Molecular Biology).
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