Measurements of astrocyte proliferation

Marina Guizzetti, Terrance J. Kavanagh, Lucio G. Costa

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Astrocytes, the most abundant cell type in the brain, proliferate during brain development. While it is generally accepted that mature astrocytes do not proliferate, neural stem cells, which have characteristics of astrocytes, retain the ability of self-renewal. Furthermore, astrocytes can regain their proliferative properties under pathological situations, such as reactive astrogliosis, a consequence of brain injury and brain tumors. Measurements of astrocyte proliferation can thus be used in investigations of physiological and pathological processes occurring in the developing and the adult brain. This chapter describes two methods for the determination of astrocyte proliferation: the incorporation of a radioactive nucleotide [3H]thymidine into DNA, which occurs during the process of DNA synthesis preceding cell division, and the flow cytometric analysis of cell cycle progression through the different phases of the cell cycle by BrDu/Hoechst and ethidium bromide labeling.

Original languageEnglish (US)
Title of host publicationIn Vitro Neurotoxicology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages349-359
Number of pages11
ISBN (Print)9781617791697
DOIs
StatePublished - Jan 1 2011

Publication series

NameMethods in Molecular Biology
Volume758
ISSN (Print)1064-3745

Keywords

  • Astrocytes
  • Bromodeoxyuridine
  • Cell cycle progression
  • Cell proliferation
  • DNA synthesis
  • Flow cytometer
  • Hoechst

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Guizzetti, M., Kavanagh, T. J., & Costa, L. G. (2011). Measurements of astrocyte proliferation. In In Vitro Neurotoxicology: Methods and Protocols (pp. 349-359). (Methods in Molecular Biology; Vol. 758). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-170-3_24