TY - JOUR
T1 - Measurement of Prostaglandins by Radioimmunoassay
AU - Campbell, William B.
AU - Ojeda, Sergio R.
N1 - Funding Information:
The authors thank Ms. Debbie Shuttlesworth for her secretarial experience. Support for the studies were provided by grants from the National Institute of Health (HL-21066 and HL-25471 to W.B.C. and HD-09988, Project IV, to S,R.O.) and a grant from the National Science Foundation (BNS-8318017 to S.R.O.). Dr. Campbell is the recipient of a Research Career Development Award from the National Institutes of Health (K04-HL-00801).
PY - 1987/1
Y1 - 1987/1
N2 - Prostaglandins (PGs) and their metabolites are measured by gas chromatography with mass spectrometric or electron capture detection, high-pressure liquid chromatography (HPLC) with ultraviolet or fluorescent detection, or radioimmunoassay. Of these methods, radioimmunoassay offers the advantages of high sensitivity, specificity, simplicity, and economy. This chapter presents a method that is applied to the measurement of PGs, PG metabolites, thromboxanes, and other eicosanoids by radioimmunoassay. It discusses the preparation of immunogenes. The PG is coupled to porcine thyroglobulin by the mixed anhydride method. Other proteins as bovine serum albumin, bovine γ globulin, or keyhole limpet hemocyanin may be used. Immunization is carried out in rabbits or chickens. Rabbits are injected with this emulsion in multiple (30–40) subcutaneous sites on the back using the same syringe and needle. Chickens are injected in the wing web, back muscles, and breast muscles. To be assured of obtaining antibodies, it is recommended that two to four animals be injected with the same immunogen. The chapter presents the characterization of antisera.
AB - Prostaglandins (PGs) and their metabolites are measured by gas chromatography with mass spectrometric or electron capture detection, high-pressure liquid chromatography (HPLC) with ultraviolet or fluorescent detection, or radioimmunoassay. Of these methods, radioimmunoassay offers the advantages of high sensitivity, specificity, simplicity, and economy. This chapter presents a method that is applied to the measurement of PGs, PG metabolites, thromboxanes, and other eicosanoids by radioimmunoassay. It discusses the preparation of immunogenes. The PG is coupled to porcine thyroglobulin by the mixed anhydride method. Other proteins as bovine serum albumin, bovine γ globulin, or keyhole limpet hemocyanin may be used. Immunization is carried out in rabbits or chickens. Rabbits are injected with this emulsion in multiple (30–40) subcutaneous sites on the back using the same syringe and needle. Chickens are injected in the wing web, back muscles, and breast muscles. To be assured of obtaining antibodies, it is recommended that two to four animals be injected with the same immunogen. The chapter presents the characterization of antisera.
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U2 - 10.1016/0076-6879(87)41080-X
DO - 10.1016/0076-6879(87)41080-X
M3 - Article
C2 - 3600362
AN - SCOPUS:0023161294
SN - 0076-6879
VL - 141
SP - 323
EP - 341
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -