Measurement of mucosal blood flow by assay of absorption of 125I from the intestinal lumen

George D. Giraud, Keith L. MacCannell

    Research output: Contribution to journalArticle

    Abstract

    A method which utilized the absorption of 125I as a measure of intestinal mucosal blood flow was developed. In the pentobarbital anesthetized dog a segment of mid-jejunum was isolated from adjacent vasculature and perfused with 125I solution. Superior mesenteric artery flow, blood flow to the isolated segment of intestine and 125I absorption were measured. Changes in 125I absorption were found to correlate closely with changes in blood flow (r = 0.95). Using an autoradiographic technique 125I absorption was found to be localized to the intestinal mucosa.

    Original languageEnglish (US)
    Pages (from-to)65-75
    Number of pages11
    JournalJournal of Pharmacological Methods
    Volume6
    Issue number1
    DOIs
    StatePublished - Aug 1981

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    Keywords

    • Blood flow
    • Gut blood flow
    • Intestinal mucosa
    • Mesenteric circulation

    ASJC Scopus subject areas

    • Pharmacology

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