TY - GEN
T1 - Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis
AU - Ortiz de Solorzano, C.
AU - Chin, K.
AU - Chou, W. S.
AU - Knowles, D.
AU - Gray, J. W.
AU - Lockett, S. J.
PY - 1999
Y1 - 1999
N2 - Genetic Instability (GI) is frequently associated with solid tumors including cancers of the breast. We hypothesize that the degree of GI, as evidenced by the cell-to-cell variations in the copy number of specific DNA loci, can be used as a marker for cancer prognosis. In this paper we present a computer-aided method for GI measurement in thick tissue sections based on confocal microscopy and 3D image analysis and visualization. The results, based on the analysis of breast tissue sections show considerable cell-to-cell variability in the copy number of the centromere of chromosome 1 and the 20q13 locus in invasive cancer but the normal 2 copies per cell in histologically normal tissue.
AB - Genetic Instability (GI) is frequently associated with solid tumors including cancers of the breast. We hypothesize that the degree of GI, as evidenced by the cell-to-cell variations in the copy number of specific DNA loci, can be used as a marker for cancer prognosis. In this paper we present a computer-aided method for GI measurement in thick tissue sections based on confocal microscopy and 3D image analysis and visualization. The results, based on the analysis of breast tissue sections show considerable cell-to-cell variability in the copy number of the centromere of chromosome 1 and the 20q13 locus in invasive cancer but the normal 2 copies per cell in histologically normal tissue.
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M3 - Conference contribution
AN - SCOPUS:0033340825
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 822
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -