Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis

C. Ortiz de Solorzano, K. Chin, W. S. Chou, D. Knowles, J. W. Gray, S. J. Lockett

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Genetic Instability (GI) is frequently associated with solid tumors including cancers of the breast. We hypothesize that the degree of GI, as evidenced by the cell-to-cell variations in the copy number of specific DNA loci, can be used as a marker for cancer prognosis. In this paper we present a computer-aided method for GI measurement in thick tissue sections based on confocal microscopy and 3D image analysis and visualization. The results, based on the analysis of breast tissue sections show considerable cell-to-cell variability in the copy number of the centromere of chromosome 1 and the 20q13 locus in invasive cancer but the normal 2 copies per cell in histologically normal tissue.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume2
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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  • Cite this

    Ortiz de Solorzano, C., Chin, K., Chou, W. S., Knowles, D., Gray, J. W., & Lockett, S. J. (1999). Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 2). IEEE.