Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis

C. Ortiz de Solorzano, Kwang-Yung Chin, W. S. Chou, D. Knowles, Joe Gray, S. J. Lockett

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Genetic Instability (GI) is frequently associated with solid tumors including cancers of the breast. We hypothesize that the degree of GI, as evidenced by the cell-to-cell variations in the copy number of specific DNA loci, can be used as a marker for cancer prognosis. In this paper we present a computer-aided method for GI measurement in thick tissue sections based on confocal microscopy and 3D image analysis and visualization. The results, based on the analysis of breast tissue sections show considerable cell-to-cell variability in the copy number of the centromere of chromosome 1 and the 20q13 locus in invasive cancer but the normal 2 copies per cell in histologically normal tissue.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Pages822
Number of pages1
Volume2
ISBN (Print)0780356756
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Confocal microscopy
Image analysis
Tissue
Chromosomes
Tumors
DNA
Visualization

ASJC Scopus subject areas

  • Bioengineering

Cite this

Ortiz de Solorzano, C., Chin, K-Y., Chou, W. S., Knowles, D., Gray, J., & Lockett, S. J. (1999). Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 2, pp. 822). IEEE.

Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis. / Ortiz de Solorzano, C.; Chin, Kwang-Yung; Chou, W. S.; Knowles, D.; Gray, Joe; Lockett, S. J.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 2 IEEE, 1999. p. 822.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ortiz de Solorzano, C, Chin, K-Y, Chou, WS, Knowles, D, Gray, J & Lockett, SJ 1999, Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 2, IEEE, pp. 822, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Ortiz de Solorzano C, Chin K-Y, Chou WS, Knowles D, Gray J, Lockett SJ. Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 2. IEEE. 1999. p. 822
Ortiz de Solorzano, C. ; Chin, Kwang-Yung ; Chou, W. S. ; Knowles, D. ; Gray, Joe ; Lockett, S. J. / Measurement of genetic instability in breast cancer by confocal microscopy and 3D image analysis. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 2 IEEE, 1999. pp. 822
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