Measurement of DNA copy number at microsatellite loci using quantitative PCR analysis

D. G. Ginzinger, T. E. Godfrey, J. Nigro, D. H. Moore, S. Suzuki, M. G. Pallavicini, J. W. Gray, R. H. Jensen

Research output: Contribution to journalArticlepeer-review

140 Scopus citations

Abstract

This report describes the development and validation of quantitative microsatellite analysis (QuMA) for rapid measurement of relative DNA sequence copy number. In QuMA, the copy number of a test locus relative to a pooled reference is assessed using quantitative, real-time PCR amplification of loci carrying simple sequence repeats. Use of simple sequence repeats is advantageous because of the large numbers that are mapped precisely. In addition, all markers are informative because QuMA does not require that they be polymorphic. The utility of QuMA is demonstrated in assessment of the extent of deletions of chromosome 2 in leukemias arising in radiation-sensitive inbred SJL mice and in analysis of the association of increased copy number of the putative oncogene ZNF217 with reduced survival duration in ovarian cancer patients.

Original languageEnglish (US)
Pages (from-to)5405-5409
Number of pages5
JournalCancer Research
Volume60
Issue number19
StatePublished - Oct 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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