Measurement of DNA antibodies

Robert (Rob) Bennett, E. Molina

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Modifications of the standard Farr technique for assaying DNA antibodies are presented; these result in improved separation of normals from abnormals, enhanced reproducibility, and simplicity of performance. The use of a 0.4 M borate buffer extends the range of the assay, while a 0.1 M buffer aids differentiation when equivocal results are obtained. Centrifugation of vials prior to counting improves the reproducibility by approximately 3%. Polyethylene glycol, at a final concentration of 6 Gm. per 100 ml., can be substituted for ammonium sulfate, with some advantages.

Original languageEnglish (US)
Pages (from-to)364-367
Number of pages4
JournalAmerican Journal of Clinical Pathology
Volume65
Issue number3
StatePublished - 1976
Externally publishedYes

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Bennett, R. R., & Molina, E. (1976). Measurement of DNA antibodies. American Journal of Clinical Pathology, 65(3), 364-367.