Measurement of absolute blood flow velocity in outflow tract of HH18 chicken embryo based on 4D reconstruction using spectral domain optical coherence tomography

Zhenhe Ma, Aiping Liu, Xin Yin, Aaron Troyer, Kent Thornburg, Ruikang K. Wang, Sandra Rugonyi

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The measurement of blood-plasma absolute velocity distributions with high spatial and temporal resolution in vivo is important for the investigation of embryonic heart at its early stage of development. We introduce a novel method to measure absolute blood flow velocity based on high speed spectral domain optical coherence tomography (OCT) and apply it to measure velocities across the heart outflow tract (OFT) of a chicken embryo (stage HH18). First, we use the OCT system to acquire 4D [(x,y,z) + t] images of the OFT in vivo. Second, we reconstruct the 4D microstructural images and obtain the orientation of the OFT at its maximum expansion, from which the centerline of the OFT is calculated based on the OFT boundary segmentation. Assuming flow is parallel to the vessel orientation, the obtained centerline indicates the flow direction. Finally, the absolute flow velocity is evaluated based on the direction given by the centerline and the axial velocity obtained from Doppler OCT. Using this method, we compare flow velocity profiles at various positions along the chicken embryo OFT.

Original languageEnglish (US)
Pages (from-to)798-811
Number of pages14
JournalBiomedical Optics Express
Volume1
Issue number3
DOIs
StatePublished - Oct 1 2010

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chickens
Blood Flow Velocity
embryos
Optical Coherence Tomography
blood flow
Chickens
Embryonic Structures
flow velocity
tomography
velocity distribution
blood plasma
parallel flow
temporal resolution
vessels
spatial resolution
high speed
expansion
high resolution
Direction compound

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Measurement of absolute blood flow velocity in outflow tract of HH18 chicken embryo based on 4D reconstruction using spectral domain optical coherence tomography. / Ma, Zhenhe; Liu, Aiping; Yin, Xin; Troyer, Aaron; Thornburg, Kent; Wang, Ruikang K.; Rugonyi, Sandra.

In: Biomedical Optics Express, Vol. 1, No. 3, 01.10.2010, p. 798-811.

Research output: Contribution to journalArticle

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