Measurement and purification of human chromosomes by flow cytometry and sorting

A. V. Carrano, Joe Gray, R. G. Langlois, K. J. Burkhart-Schultz, M. A. Van Dilla

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The 24 human chromosome types of a normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophotometry of metaphase chromosomes from the same cell strain supported and extended this identification. Four of the groups purified were due to chromosomes of a single type - namely, chromosomes 5, 6, 13, and 17. Eight additional groups were also separated and found to contain the following chromosomes: 1 and 2; 3 and 4; 7, 8, and X; 9-12; 14 and 15; 16 and 18; 20 and Y; and 19, 21, and 22. The average purity for the 12 sorted fractions was 78%.

Original languageEnglish (US)
Pages (from-to)1382-1384
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume76
Issue number3
StatePublished - 1979
Externally publishedYes

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Human Chromosomes
Flow Cytometry
Chromosomes
Cytophotometry
Bisbenzimidazole
Quinacrine
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 2
Chromosomes, Human, Pair 1
Metaphase
Diploidy
Fibroblasts
Fluorescence
DNA

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Measurement and purification of human chromosomes by flow cytometry and sorting. / Carrano, A. V.; Gray, Joe; Langlois, R. G.; Burkhart-Schultz, K. J.; Van Dilla, M. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, No. 3, 1979, p. 1382-1384.

Research output: Contribution to journalArticle

Carrano, A. V. ; Gray, Joe ; Langlois, R. G. ; Burkhart-Schultz, K. J. ; Van Dilla, M. A. / Measurement and purification of human chromosomes by flow cytometry and sorting. In: Proceedings of the National Academy of Sciences of the United States of America. 1979 ; Vol. 76, No. 3. pp. 1382-1384.
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