Measurement and purification of human chromosomes by flow cytometry and sorting

A. V. Carrano, J. W. Gray, R. G. Langlois, K. J. Burkhart-Schultz, M. A. Van Dilla

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Abstract

The 24 human chromosome types of a normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophotometry of metaphase chromosomes from the same cell strain supported and extended this identification. Four of the groups purified were due to chromosomes of a single type - namely, chromosomes 5, 6, 13, and 17. Eight additional groups were also separated and found to contain the following chromosomes: 1 and 2; 3 and 4; 7, 8, and X; 9-12; 14 and 15; 16 and 18; 20 and Y; and 19, 21, and 22. The average purity for the 12 sorted fractions was 78%.

Original languageEnglish (US)
Pages (from-to)1382-1384
Number of pages3
JournalUnknown Journal
Volume76
Issue number3
DOIs
StatePublished - 1979

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    Carrano, A. V., Gray, J. W., Langlois, R. G., Burkhart-Schultz, K. J., & Van Dilla, M. A. (1979). Measurement and purification of human chromosomes by flow cytometry and sorting. Unknown Journal, 76(3), 1382-1384. https://doi.org/10.1073/pnas.76.3.1382