Abstract
The 24 human chromosome types of a normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophotometry of metaphase chromosomes from the same cell strain supported and extended this identification. Four of the groups purified were due to chromosomes of a single type - namely, chromosomes 5, 6, 13, and 17. Eight additional groups were also separated and found to contain the following chromosomes: 1 and 2; 3 and 4; 7, 8, and X; 9-12; 14 and 15; 16 and 18; 20 and Y; and 19, 21, and 22. The average purity for the 12 sorted fractions was 78%.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1382-1384 |
| Number of pages | 3 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 76 |
| Issue number | 3 |
| State | Published - 1979 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- General
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Measurement and purification of human chromosomes by flow cytometry and sorting. / Carrano, A. V.; Gray, Joe; Langlois, R. G.; Burkhart-Schultz, K. J.; Van Dilla, M. A.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, No. 3, 1979, p. 1382-1384.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement and purification of human chromosomes by flow cytometry and sorting
AU - Carrano, A. V.
AU - Gray, Joe
AU - Langlois, R. G.
AU - Burkhart-Schultz, K. J.
AU - Van Dilla, M. A.
PY - 1979
Y1 - 1979
N2 - The 24 human chromosome types of a normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophotometry of metaphase chromosomes from the same cell strain supported and extended this identification. Four of the groups purified were due to chromosomes of a single type - namely, chromosomes 5, 6, 13, and 17. Eight additional groups were also separated and found to contain the following chromosomes: 1 and 2; 3 and 4; 7, 8, and X; 9-12; 14 and 15; 16 and 18; 20 and Y; and 19, 21, and 22. The average purity for the 12 sorted fractions was 78%.
AB - The 24 human chromosome types of a normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophotometry of metaphase chromosomes from the same cell strain supported and extended this identification. Four of the groups purified were due to chromosomes of a single type - namely, chromosomes 5, 6, 13, and 17. Eight additional groups were also separated and found to contain the following chromosomes: 1 and 2; 3 and 4; 7, 8, and X; 9-12; 14 and 15; 16 and 18; 20 and Y; and 19, 21, and 22. The average purity for the 12 sorted fractions was 78%.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018344952&partnerID=8YFLogxK
M3 - Article
C2 - 286320
AN - SCOPUS:0018344952
VL - 76
SP - 1382
EP - 1384
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 3
ER -