Abstract
The existence and distribution of angiotensin I (A I) and angiotensin II (A II) in rat kidney were examined in immunocytochemical studies using the peroxidase-antiperoxidase technique and in biochemical studies using rat kidney homogenates extracted with acid-ethanol and purified by Sephadex G-25 gel chromatography. Immunopositive A II-like staining was observed in the juxtaglomerular cells of the afferent arteriole, but no histochemical evidence for A I was found. On the other hand, renal homogenates were found to contain both A I and A II immunoreactivities which coeluted on gel chromatography with synthetic A I and A II. These results indicate that A I as well as A II immunoreactivities are present in the kidney and that A II immunoreactivity can be localized to the juxtaglomerular cells. The origin of the immunoreactive A II in the juxtaglomerular cells remains to be determined.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 317-324 |
| Number of pages | 8 |
| Journal | Regulatory Peptides |
| Volume | 4 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 1982 |
Keywords
- angiotensin I
- angiotensin II
- intrarenal renin-angiotensin system
- peroxidase-antiperoxidase immunohistochemistry
- renin
- tubulo-glomerular feedback
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Endocrinology
- Clinical Biochemistry
- Cellular and Molecular Neuroscience