Measurement and localization of angiotensin-like immunoreactivity in juxtaglomerular cells of the rat

Virginia Brooks, Mark S. Brownfield, Ian A. Reid

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The existence and distribution of angiotensin I (A I) and angiotensin II (A II) in rat kidney were examined in immunocytochemical studies using the peroxidase-antiperoxidase technique and in biochemical studies using rat kidney homogenates extracted with acid-ethanol and purified by Sephadex G-25 gel chromatography. Immunopositive A II-like staining was observed in the juxtaglomerular cells of the afferent arteriole, but no histochemical evidence for A I was found. On the other hand, renal homogenates were found to contain both A I and A II immunoreactivities which coeluted on gel chromatography with synthetic A I and A II. These results indicate that A I as well as A II immunoreactivities are present in the kidney and that A II immunoreactivity can be localized to the juxtaglomerular cells. The origin of the immunoreactive A II in the juxtaglomerular cells remains to be determined.

Original languageEnglish (US)
Pages (from-to)317-324
Number of pages8
JournalRegulatory Peptides
Volume4
Issue number6
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Angiotensins
Angiotensin II
Angiotensin I
Rats
Cells
Kidney
Chromatography
Gel Chromatography
Gels
Arterioles
Peroxidase
Ethanol
Staining and Labeling
Acids

Keywords

  • angiotensin I
  • angiotensin II
  • intrarenal renin-angiotensin system
  • peroxidase-antiperoxidase immunohistochemistry
  • renin
  • tubulo-glomerular feedback

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Measurement and localization of angiotensin-like immunoreactivity in juxtaglomerular cells of the rat. / Brooks, Virginia; Brownfield, Mark S.; Reid, Ian A.

In: Regulatory Peptides, Vol. 4, No. 6, 1982, p. 317-324.

Research output: Contribution to journalArticle

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abstract = "The existence and distribution of angiotensin I (A I) and angiotensin II (A II) in rat kidney were examined in immunocytochemical studies using the peroxidase-antiperoxidase technique and in biochemical studies using rat kidney homogenates extracted with acid-ethanol and purified by Sephadex G-25 gel chromatography. Immunopositive A II-like staining was observed in the juxtaglomerular cells of the afferent arteriole, but no histochemical evidence for A I was found. On the other hand, renal homogenates were found to contain both A I and A II immunoreactivities which coeluted on gel chromatography with synthetic A I and A II. These results indicate that A I as well as A II immunoreactivities are present in the kidney and that A II immunoreactivity can be localized to the juxtaglomerular cells. The origin of the immunoreactive A II in the juxtaglomerular cells remains to be determined.",
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AU - Reid, Ian A.

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N2 - The existence and distribution of angiotensin I (A I) and angiotensin II (A II) in rat kidney were examined in immunocytochemical studies using the peroxidase-antiperoxidase technique and in biochemical studies using rat kidney homogenates extracted with acid-ethanol and purified by Sephadex G-25 gel chromatography. Immunopositive A II-like staining was observed in the juxtaglomerular cells of the afferent arteriole, but no histochemical evidence for A I was found. On the other hand, renal homogenates were found to contain both A I and A II immunoreactivities which coeluted on gel chromatography with synthetic A I and A II. These results indicate that A I as well as A II immunoreactivities are present in the kidney and that A II immunoreactivity can be localized to the juxtaglomerular cells. The origin of the immunoreactive A II in the juxtaglomerular cells remains to be determined.

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