Abstract
The existence and distribution of angiotensin I (A I) and angiotensin II (A II) in rat kidney were examined in immunocytochemical studies using the peroxidase-antiperoxidase technique and in biochemical studies using rat kidney homogenates extracted with acid-ethanol and purified by Sephadex G-25 gel chromatography. Immunopositive A II-like staining was observed in the juxtaglomerular cells of the afferent arteriole, but no histochemical evidence for A I was found. On the other hand, renal homogenates were found to contain both A I and A II immunoreactivities which coeluted on gel chromatography with synthetic A I and A II. These results indicate that A I as well as A II immunoreactivities are present in the kidney and that A II immunoreactivity can be localized to the juxtaglomerular cells. The origin of the immunoreactive A II in the juxtaglomerular cells remains to be determined.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 317-324 |
| Number of pages | 8 |
| Journal | Regulatory Peptides |
| Volume | 4 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1982 |
| Externally published | Yes |
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Keywords
- angiotensin I
- angiotensin II
- intrarenal renin-angiotensin system
- peroxidase-antiperoxidase immunohistochemistry
- renin
- tubulo-glomerular feedback
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
- Neuroscience(all)
Cite this
Measurement and localization of angiotensin-like immunoreactivity in juxtaglomerular cells of the rat. / Brooks, Virginia; Brownfield, Mark S.; Reid, Ian A.
In: Regulatory Peptides, Vol. 4, No. 6, 1982, p. 317-324.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement and localization of angiotensin-like immunoreactivity in juxtaglomerular cells of the rat
AU - Brooks, Virginia
AU - Brownfield, Mark S.
AU - Reid, Ian A.
PY - 1982
Y1 - 1982
N2 - The existence and distribution of angiotensin I (A I) and angiotensin II (A II) in rat kidney were examined in immunocytochemical studies using the peroxidase-antiperoxidase technique and in biochemical studies using rat kidney homogenates extracted with acid-ethanol and purified by Sephadex G-25 gel chromatography. Immunopositive A II-like staining was observed in the juxtaglomerular cells of the afferent arteriole, but no histochemical evidence for A I was found. On the other hand, renal homogenates were found to contain both A I and A II immunoreactivities which coeluted on gel chromatography with synthetic A I and A II. These results indicate that A I as well as A II immunoreactivities are present in the kidney and that A II immunoreactivity can be localized to the juxtaglomerular cells. The origin of the immunoreactive A II in the juxtaglomerular cells remains to be determined.
AB - The existence and distribution of angiotensin I (A I) and angiotensin II (A II) in rat kidney were examined in immunocytochemical studies using the peroxidase-antiperoxidase technique and in biochemical studies using rat kidney homogenates extracted with acid-ethanol and purified by Sephadex G-25 gel chromatography. Immunopositive A II-like staining was observed in the juxtaglomerular cells of the afferent arteriole, but no histochemical evidence for A I was found. On the other hand, renal homogenates were found to contain both A I and A II immunoreactivities which coeluted on gel chromatography with synthetic A I and A II. These results indicate that A I as well as A II immunoreactivities are present in the kidney and that A II immunoreactivity can be localized to the juxtaglomerular cells. The origin of the immunoreactive A II in the juxtaglomerular cells remains to be determined.
KW - angiotensin I
KW - angiotensin II
KW - intrarenal renin-angiotensin system
KW - peroxidase-antiperoxidase immunohistochemistry
KW - renin
KW - tubulo-glomerular feedback
UR - http://www.scopus.com/inward/record.url?scp=0020379344&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020379344&partnerID=8YFLogxK
U2 - 10.1016/0167-0115(82)90142-2
DO - 10.1016/0167-0115(82)90142-2
M3 - Article
C2 - 6758058
AN - SCOPUS:0020379344
VL - 4
SP - 317
EP - 324
JO - Regulatory Peptides
JF - Regulatory Peptides
SN - 0167-0115
IS - 6
ER -