Measles virus-induced immunosuppression in vitro is associated with deregulation of G1 cell cycle control proteins

Oliver Engelking, Lev Fedorov, Richard Lilischkis, Volker Ter Meulen, Sibylle Schneider-Schaulies

Research output: Contribution to journalArticle

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Abstract

Virus-induced immunosuppression is the major cause of the high morbidity/mortality rates associated with acute measles. It has been shown previously that mitogen-dependent proliferation of peripheral blood lymphocytes (PBL) was strongly impaired after contact with the measles virus (MV) glycoproteins F and H expressed on the surface of infected cells, cells transfected with the corresponding expression constructs or UV-inactivated MV (UV-MV). The state of unresponsiveness was not associated with the induction of apoptosis, and a significant proportion of PBL was found to be arrested in the G0/G1 phase of the cell cycle. It is now shown that cell cycle cessation, rather than complete arrest, is induced after MV glycoprotein contact. No obvious role was found for p53 in the induction of this unresponsiveness. With UV-MV as effector, downregulation of p27, an inhibitor of cyclin-dependent kinase (CDK)-cyclin complexes, was significantly delayed after mitogenic stimulation of human PBL. The activities of both CDK4/6-cyclin D and CDK2-cyclin E complexes for phosphorylation of exogenous substrates in vitro were strongly reduced. CDK4, CDK6, cyclins D3 and E and, to a minor extent, CDK2 failed to accumulate at the protein level after mitogenic stimulation in the presence of UV-MV. These data indicate that MV-induced proliferative unresponsiveness of PBL to mitogenic stimulation is associated with a drastic deregulation of the expression of cell cycle genes essential for the G1/S phase transition.

Original languageEnglish (US)
Pages (from-to)1599-1608
Number of pages10
JournalJournal of General Virology
Volume80
Issue number7
StatePublished - 1999
Externally publishedYes

Fingerprint

Cell Cycle Proteins
Measles virus
Cell Cycle Checkpoints
Immunosuppression
Lymphocytes
Cyclin E
G1 Phase
Glycoproteins
Cell Cycle
Cyclin D3
Cyclin D
cdc Genes
Cell Cycle Resting Phase
Cyclins
Cyclin-Dependent Kinases
Phase Transition
Measles
S Phase
Mitogens
Down-Regulation

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Engelking, O., Fedorov, L., Lilischkis, R., Ter Meulen, V., & Schneider-Schaulies, S. (1999). Measles virus-induced immunosuppression in vitro is associated with deregulation of G1 cell cycle control proteins. Journal of General Virology, 80(7), 1599-1608.

Measles virus-induced immunosuppression in vitro is associated with deregulation of G1 cell cycle control proteins. / Engelking, Oliver; Fedorov, Lev; Lilischkis, Richard; Ter Meulen, Volker; Schneider-Schaulies, Sibylle.

In: Journal of General Virology, Vol. 80, No. 7, 1999, p. 1599-1608.

Research output: Contribution to journalArticle

Engelking, O, Fedorov, L, Lilischkis, R, Ter Meulen, V & Schneider-Schaulies, S 1999, 'Measles virus-induced immunosuppression in vitro is associated with deregulation of G1 cell cycle control proteins', Journal of General Virology, vol. 80, no. 7, pp. 1599-1608.
Engelking, Oliver ; Fedorov, Lev ; Lilischkis, Richard ; Ter Meulen, Volker ; Schneider-Schaulies, Sibylle. / Measles virus-induced immunosuppression in vitro is associated with deregulation of G1 cell cycle control proteins. In: Journal of General Virology. 1999 ; Vol. 80, No. 7. pp. 1599-1608.
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