Matrix metalloproteinase expression in Macaca mulatta endometrium: Evidence for zone-specific regulatory tissue gradients

Laura A. Rudolph-Owen, Ov Slayden, Lynn M. Matrisian, Robert M. Brenner

    Research output: Contribution to journalArticle

    74 Citations (Scopus)

    Abstract

    Matrix metalloproteinases (MMPs) are highly expressed in the human endometrium during menstruation, and these enzymes participate in the cyclic destruction and regeneration characteristic of the primate endometrium. To examine hormonal regulation of MMPs in vivo, we evaluated MMP expression and localization in the endometrium of ovariectomized rhesus macaques under various hormonal conditions. Although all MMPs were up-regulated by progesterone (P4) withdrawal, their expression declined spontaneously after menstruation in the absence of P4. Of 7 MMPs examined, only matrilysin and stromelysin-3 were suppressed any further when P4 levels were experimentally re-elevated. MMP expression was confined to the upper functionalis zone during menstruation, but after menstrual breakdown was complete, matrilysin and the tissue inhibitor of MMPs, TIMP-1, shifted expression from the functionalis to the basalis zone in the absence of both estradiol and P4. The spiral arteries in the functionalis, but not the basalis, were intense foci of MMP and TIMP-1 expression. Menstruation and MMP expression after P4 withdrawal were similar in both the presence and absence of estradiol. In sum, endometrial MMPs in vivo are strongly up-regulated by P4 withdrawal, but zone-specific tissue gradients greatly influence the pattern and degree of MMP expression.

    Original languageEnglish (US)
    Pages (from-to)1349-1359
    Number of pages11
    JournalBiology of Reproduction
    Volume59
    Issue number6
    StatePublished - Dec 1998

    Fingerprint

    Endometrium
    Macaca mulatta
    Matrix Metalloproteinases
    Menstruation
    Matrix Metalloproteinase 7
    Matrix Metalloproteinase 1
    Tissue Inhibitor of Metalloproteinase-1
    Estradiol
    Matrix Metalloproteinase 11
    Tissue Inhibitor of Metalloproteinases
    Primates
    Progesterone
    Regeneration
    Arteries
    Enzymes

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Matrix metalloproteinase expression in Macaca mulatta endometrium : Evidence for zone-specific regulatory tissue gradients. / Rudolph-Owen, Laura A.; Slayden, Ov; Matrisian, Lynn M.; Brenner, Robert M.

    In: Biology of Reproduction, Vol. 59, No. 6, 12.1998, p. 1349-1359.

    Research output: Contribution to journalArticle

    Rudolph-Owen, Laura A. ; Slayden, Ov ; Matrisian, Lynn M. ; Brenner, Robert M. / Matrix metalloproteinase expression in Macaca mulatta endometrium : Evidence for zone-specific regulatory tissue gradients. In: Biology of Reproduction. 1998 ; Vol. 59, No. 6. pp. 1349-1359.
    @article{d066135594614782ad5020c88d0b3217,
    title = "Matrix metalloproteinase expression in Macaca mulatta endometrium: Evidence for zone-specific regulatory tissue gradients",
    abstract = "Matrix metalloproteinases (MMPs) are highly expressed in the human endometrium during menstruation, and these enzymes participate in the cyclic destruction and regeneration characteristic of the primate endometrium. To examine hormonal regulation of MMPs in vivo, we evaluated MMP expression and localization in the endometrium of ovariectomized rhesus macaques under various hormonal conditions. Although all MMPs were up-regulated by progesterone (P4) withdrawal, their expression declined spontaneously after menstruation in the absence of P4. Of 7 MMPs examined, only matrilysin and stromelysin-3 were suppressed any further when P4 levels were experimentally re-elevated. MMP expression was confined to the upper functionalis zone during menstruation, but after menstrual breakdown was complete, matrilysin and the tissue inhibitor of MMPs, TIMP-1, shifted expression from the functionalis to the basalis zone in the absence of both estradiol and P4. The spiral arteries in the functionalis, but not the basalis, were intense foci of MMP and TIMP-1 expression. Menstruation and MMP expression after P4 withdrawal were similar in both the presence and absence of estradiol. In sum, endometrial MMPs in vivo are strongly up-regulated by P4 withdrawal, but zone-specific tissue gradients greatly influence the pattern and degree of MMP expression.",
    author = "Rudolph-Owen, {Laura A.} and Ov Slayden and Matrisian, {Lynn M.} and Brenner, {Robert M.}",
    year = "1998",
    month = "12",
    language = "English (US)",
    volume = "59",
    pages = "1349--1359",
    journal = "Biology of Reproduction",
    issn = "0006-3363",
    publisher = "Society for the Study of Reproduction",
    number = "6",

    }

    TY - JOUR

    T1 - Matrix metalloproteinase expression in Macaca mulatta endometrium

    T2 - Evidence for zone-specific regulatory tissue gradients

    AU - Rudolph-Owen, Laura A.

    AU - Slayden, Ov

    AU - Matrisian, Lynn M.

    AU - Brenner, Robert M.

    PY - 1998/12

    Y1 - 1998/12

    N2 - Matrix metalloproteinases (MMPs) are highly expressed in the human endometrium during menstruation, and these enzymes participate in the cyclic destruction and regeneration characteristic of the primate endometrium. To examine hormonal regulation of MMPs in vivo, we evaluated MMP expression and localization in the endometrium of ovariectomized rhesus macaques under various hormonal conditions. Although all MMPs were up-regulated by progesterone (P4) withdrawal, their expression declined spontaneously after menstruation in the absence of P4. Of 7 MMPs examined, only matrilysin and stromelysin-3 were suppressed any further when P4 levels were experimentally re-elevated. MMP expression was confined to the upper functionalis zone during menstruation, but after menstrual breakdown was complete, matrilysin and the tissue inhibitor of MMPs, TIMP-1, shifted expression from the functionalis to the basalis zone in the absence of both estradiol and P4. The spiral arteries in the functionalis, but not the basalis, were intense foci of MMP and TIMP-1 expression. Menstruation and MMP expression after P4 withdrawal were similar in both the presence and absence of estradiol. In sum, endometrial MMPs in vivo are strongly up-regulated by P4 withdrawal, but zone-specific tissue gradients greatly influence the pattern and degree of MMP expression.

    AB - Matrix metalloproteinases (MMPs) are highly expressed in the human endometrium during menstruation, and these enzymes participate in the cyclic destruction and regeneration characteristic of the primate endometrium. To examine hormonal regulation of MMPs in vivo, we evaluated MMP expression and localization in the endometrium of ovariectomized rhesus macaques under various hormonal conditions. Although all MMPs were up-regulated by progesterone (P4) withdrawal, their expression declined spontaneously after menstruation in the absence of P4. Of 7 MMPs examined, only matrilysin and stromelysin-3 were suppressed any further when P4 levels were experimentally re-elevated. MMP expression was confined to the upper functionalis zone during menstruation, but after menstrual breakdown was complete, matrilysin and the tissue inhibitor of MMPs, TIMP-1, shifted expression from the functionalis to the basalis zone in the absence of both estradiol and P4. The spiral arteries in the functionalis, but not the basalis, were intense foci of MMP and TIMP-1 expression. Menstruation and MMP expression after P4 withdrawal were similar in both the presence and absence of estradiol. In sum, endometrial MMPs in vivo are strongly up-regulated by P4 withdrawal, but zone-specific tissue gradients greatly influence the pattern and degree of MMP expression.

    UR - http://www.scopus.com/inward/record.url?scp=0031737949&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0031737949&partnerID=8YFLogxK

    M3 - Article

    C2 - 9828178

    AN - SCOPUS:0031737949

    VL - 59

    SP - 1349

    EP - 1359

    JO - Biology of Reproduction

    JF - Biology of Reproduction

    SN - 0006-3363

    IS - 6

    ER -