Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia

Juha Rasanen, Matthew J. Quinn, Amber Laurie, Eric Bean, Charles T. Roberts, Srinivasa R. Nagalla, Michael G. Gravett

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Objective We assessed the association of glycosylated fibronectin (GlyFn) with preeclampsia and its performance in a point-of-care (POC) test.

Study Design GlyFn, placental growth factor (PlGF), and soluble vascular endothelial growth factor receptor 1 (sFlt1) levels were determined in serum samples from 107 pregnant women. In all, 45 were normotensive and 62 were diagnosed with preeclampsia. The ability of GlyFn to assess preeclampsia status and relationships between GlyFn and maternal characteristics and pregnancy outcomes were analyzed.

Results GlyFn serum levels in the first trimester were significantly higher in women with preeclampsia (P <.01) and remained higher throughout pregnancy (P <.01). GlyFn, sFlt1, PlGF, and the sFlt1/PlGF ratio were significantly associated (P <.01) with preeclampsia status, and the classification performance of these analytes represented by area under the receiver operating characteristic curve was 0.99, 0.96, 0.94, and 0.98, respectively, with 95% confidence intervals of 0.98-1.00, 0.89-1.00, 0.86-1.00, and 0.94-1.00, respectively. Increased GlyFn levels were significantly associated with gestational age at delivery (P <.01), blood pressure (P =.04), and small-for-gestational-age neonates. Repeated-measures analysis of the difference in weekly GlyFn change in the third trimester demonstrated that mild preeclampsia was associated with a weekly change of 81.7 μg/mL (SE 94.1) vs 195.2 μg/mL (SE 88.2) for severe preeclampsia. The GlyFn POC demonstrated similar performance to a plate assay with an area under the receiver operating characteristic curve of 0.93 and 95% confidence interval of 0.85-1.00.

Conclusion GlyFn is a robust biomarker for monitoring of preeclampsia in both a standard and POC format, which supports its utility in diverse settings.

Original languageEnglish (US)
Pages (from-to)82.e1-82.e9
JournalAmerican journal of obstetrics and gynecology
Volume212
Issue number1
DOIs
StatePublished - Jan 1 2015

Keywords

  • biomarker
  • glycosylated fibronectin
  • point-of-care
  • preeclampsia

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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