Maternal high fat diet is associated with decreased plasma n-3 fatty acids and fetal hepatic apoptosis in nonhuman primates

Wilmon F. Grant, Melanie Gillingham, Ayesha K. Batra, Natasha M. Fewkes, Sarah M. Comstock, Diana Takahashi, Theodore P. Braun, Kevin Grove, Jacob E. Friedman, Daniel Marks

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

To begin to understand the contributions of maternal obesity and over-nutrition to human development and the early origins of obesity, we utilized a non-human primate model to investigate the effects of maternal high-fat feeding and obesity on breast milk, maternal and fetal plasma fatty acid composition and fetal hepatic development. While the high-fat diet (HFD) contained equivalent levels of n-3 fatty acids (FA's) and higher levels of n-6 FA's than the control diet (CTR), we found significant decreases in docosahexaenoic acid (DHA) and total n-3 FA's in HFD maternal and fetal plasma. Furthermore, the HFD fetal plasma n-6:n-3 ratio was elevated and was significantly correlated to the maternal plasma n-6:n-3 ratio and maternal hyperinsulinemia. Hepatic apoptosis was also increased in the HFD fetal liver. Switching HFD females to a CTR diet during a subsequent pregnancy normalized fetal DHA, n-3 FA's and fetal hepatic apoptosis to CTR levels. Breast milk from HFD dams contained lower levels of eicosopentanoic acid (EPA) and DHA and lower levels of total protein than CTR breast milk. This study links chronic maternal consumption of a HFD with fetal hepatic apoptosis and suggests that a potentially pathological maternal fatty acid milieu is replicated in the developing fetal circulation in the nonhuman primate.

Original languageEnglish (US)
Article numbere17261
JournalPLoS One
Volume6
Issue number2
DOIs
StatePublished - 2011

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Omega-3 Fatty Acids
High Fat Diet
high fat diet
Nutrition
omega-3 fatty acids
Primates
apoptosis
Fats
Mothers
Apoptosis
Plasmas
liver
Liver
breast milk
Docosahexaenoic Acids
docosahexaenoic acid
Diet
Human Milk
obesity
diet

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Maternal high fat diet is associated with decreased plasma n-3 fatty acids and fetal hepatic apoptosis in nonhuman primates. / Grant, Wilmon F.; Gillingham, Melanie; Batra, Ayesha K.; Fewkes, Natasha M.; Comstock, Sarah M.; Takahashi, Diana; Braun, Theodore P.; Grove, Kevin; Friedman, Jacob E.; Marks, Daniel.

In: PLoS One, Vol. 6, No. 2, e17261, 2011.

Research output: Contribution to journalArticle

Grant, Wilmon F. ; Gillingham, Melanie ; Batra, Ayesha K. ; Fewkes, Natasha M. ; Comstock, Sarah M. ; Takahashi, Diana ; Braun, Theodore P. ; Grove, Kevin ; Friedman, Jacob E. ; Marks, Daniel. / Maternal high fat diet is associated with decreased plasma n-3 fatty acids and fetal hepatic apoptosis in nonhuman primates. In: PLoS One. 2011 ; Vol. 6, No. 2.
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