Mast cells produce novel shorter forms of perlecan that contain functional endorepellin a role in angiogenesis and wound healing

Moonsun Jung, Megan S. Lord, Bill Cheng, J. Guy Lyons, Hatem Alkhouri, J. Margaret Hughes, Simon McCarthy, Renato V. Iozzo, John M. Whitelock

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Mast cells are derived from hematopoietic progenitors that are known to migrate to and reside within connective and mucosal tissues, where they differentiate and respond to various stimuli by releasing pro-inflammatory mediators, including histamine, growth factors, and proteases. This study demonstrated that primary human mast cells as well as the rat and human mast cell lines, RBL-2H3 and HMC-1, produce the heparan sulfate proteoglycan, perlecan, with a molecular mass of 640 kDa as well as smaller molecular mass species of 300 and 130 kDa. Utilizing domain-specific antibodies coupled with N-terminal sequencing, it was confirmed that both forms contained the C-terminal module of the protein core known as endorepellin, which were generated by mast cell-derived proteases. Domain-specific RT-PCR experiments demonstrated that transcripts corresponding to domains I and V, including endorepellin, were present; however, mRNA transcripts corresponding to regions of domain III were not present, suggesting that these cells were capable of producing spliced forms of the protein core. Fractions from mast cell cultures that were enriched for these fragments were shown to bind endothelial cells via the α2β1 integrin and stimulate the migration of cells in "scratch assays," both activities of which were inhibited by incubation with either anti-endorepellin or anti-perlecan antibodies. This study shows for the first time that mast cells secrete and process the extracellular proteoglycan perlecan into fragments containing the endorepellin C-terminal region that regulate angiogenesis and matrix turnover, which are both key events in wound healing.

Original languageEnglish (US)
Pages (from-to)3289-3304
Number of pages16
JournalJournal of Biological Chemistry
Volume288
Issue number5
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

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Mast Cells
Wound Healing
Molecular mass
Peptide Hydrolases
Cells
Heparan Sulfate Proteoglycans
Antibodies
Endothelial cells
Proteoglycans
Cell culture
Integrins
Histamine
Rats
Assays
Intercellular Signaling Peptides and Proteins
Proteins
Messenger RNA
Connective Tissue
Cell Movement
perlecan

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Mast cells produce novel shorter forms of perlecan that contain functional endorepellin a role in angiogenesis and wound healing. / Jung, Moonsun; Lord, Megan S.; Cheng, Bill; Lyons, J. Guy; Alkhouri, Hatem; Hughes, J. Margaret; McCarthy, Simon; Iozzo, Renato V.; Whitelock, John M.

In: Journal of Biological Chemistry, Vol. 288, No. 5, 01.02.2013, p. 3289-3304.

Research output: Contribution to journalArticle

Jung, M, Lord, MS, Cheng, B, Lyons, JG, Alkhouri, H, Hughes, JM, McCarthy, S, Iozzo, RV & Whitelock, JM 2013, 'Mast cells produce novel shorter forms of perlecan that contain functional endorepellin a role in angiogenesis and wound healing', Journal of Biological Chemistry, vol. 288, no. 5, pp. 3289-3304. https://doi.org/10.1074/jbc.M112.387811
Jung, Moonsun ; Lord, Megan S. ; Cheng, Bill ; Lyons, J. Guy ; Alkhouri, Hatem ; Hughes, J. Margaret ; McCarthy, Simon ; Iozzo, Renato V. ; Whitelock, John M. / Mast cells produce novel shorter forms of perlecan that contain functional endorepellin a role in angiogenesis and wound healing. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 5. pp. 3289-3304.
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