Mast cells in human optic nerve

L. A. Levin, Daniel Albert, D. Johnson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose. Mast cells are classically found in ocular tissues within the conjunctiva, choroid, and iris. The aim of this study was to examine their distribution in the optic nerve and its meninges. Methods. Sixty-six human optic nerves were studied from normal subjects at autopsy, fetuses aborted for chromosomal abnormalities, and from enucleation specimens of patients with a variety of inflammatory, traumatic, neoplastic, and vascular disorders. Mast cells were identified using a stain for the enzyme chloracetate esterase, and confirmed using toluidine blue, revealing metachromatic cytoplasmic granules. Results. Mast cells were found scattered in the meninges of almost all optic nerves examined, frequently in perivascular locations, with densities up to 2325 mast cells/mm3 (mean 269.7 ± 64.1 cells/mm3 in normal nerves). Mast cells were found in the optic nerve parenchyma in nerves from four eyes that had severe abnormality, often associated with neovascularization. Normal nerves, as well as nerves from fetuses aborted for congenital defects, had significantly fewer meningeal mast cells than those from eyes with inflammatory or vascular diseases. Degranulation of mast cells was observed more often in eyes with recent severe trauma. Conclusions. Based on their work and the work of others suggesting an association between mast cells and nervous system autoimmune disorders, the authors hypothesize a role for optic nerve mast cells in certain ocular inflammatory conditions.

Original languageEnglish (US)
Pages (from-to)3147-3153
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume34
Issue number11
StatePublished - Oct 27 1993
Externally publishedYes

Fingerprint

Optic Nerve
Mast Cells
Aborted Fetus
Meninges
Autoimmune Diseases of the Nervous System
Cytoplasmic Granules
Tolonium Chloride
Choroid
Conjunctiva
Iris
Esterases
Vascular Diseases
Chromosome Aberrations
Blood Vessels
Autopsy
Coloring Agents
Neurons
Wounds and Injuries
Enzymes

Keywords

  • chloroacetate esterase
  • mast cell
  • meninges
  • optic nerve
  • optic neuritis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Mast cells in human optic nerve. / Levin, L. A.; Albert, Daniel; Johnson, D.

In: Investigative Ophthalmology and Visual Science, Vol. 34, No. 11, 27.10.1993, p. 3147-3153.

Research output: Contribution to journalArticle

Levin, LA, Albert, D & Johnson, D 1993, 'Mast cells in human optic nerve', Investigative Ophthalmology and Visual Science, vol. 34, no. 11, pp. 3147-3153.
Levin, L. A. ; Albert, Daniel ; Johnson, D. / Mast cells in human optic nerve. In: Investigative Ophthalmology and Visual Science. 1993 ; Vol. 34, No. 11. pp. 3147-3153.
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