Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation

Shiying Jin, Lei Lei, Lonnie D. Shea, Mary Zelinski, Richard Stouffer, Teresa K. Woodruff

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Objective: To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices. Design: Fresh monkey ovarian tissue was frozen by slow cryopreservation and thawed for analysis of morphologic and functional parameters. Setting: University-affiliated laboratory. Animals: Rhesus monkey ovarian tissue. Intervention(s): None. Main Outcome Measure(s): Histologic analysis, follicle counting, assessment of protein abundance and localization. Result(s): After freezing and thawing, 89% of the primordial follicles maintained their laminar-based architecture, with sizes close to those of fresh fixed follicles. Molecular markers of early follicle health (activin subunits and the phosphorylated form of the signaling protein Smad2 [pSmad2]) were present in fresh and frozen-thawed primordial follicles. Stroma cells, but not follicles, had a higher level of TUNEL staining. Granulosa cells within the follicles of frozen-thawed ovarian tissue cultured for 48 hours had the capacity to proliferate and sustained expression of the activin subunits and nuclear pSmad2. Conclusion(s): This study provides evidence that markers of early follicle growth and development are preserved after slow cryopreservation and thaw, with little effect on follicle morphology and function.

    Original languageEnglish (US)
    Pages (from-to)2627-2632
    Number of pages6
    JournalFertility and Sterility
    Volume93
    Issue number8
    DOIs
    StatePublished - May 15 2010

    Fingerprint

    Cryopreservation
    Growth and Development
    Primates
    Activins
    Smad2 Protein
    Ovarian Follicle
    Granulosa Cells
    In Situ Nick-End Labeling
    Nuclear Proteins
    Macaca mulatta
    Freezing
    Haplorhini
    Outcome Assessment (Health Care)
    Staining and Labeling
    Health
    Proteins

    Keywords

    • activin subunits
    • primordial follicles
    • pSmad2
    • rhesus monkey
    • Slow cryopreservation
    • stromal cell

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Reproductive Medicine

    Cite this

    Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation. / Jin, Shiying; Lei, Lei; Shea, Lonnie D.; Zelinski, Mary; Stouffer, Richard; Woodruff, Teresa K.

    In: Fertility and Sterility, Vol. 93, No. 8, 15.05.2010, p. 2627-2632.

    Research output: Contribution to journalArticle

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