Markers of cochlear inflammation using MRI

Johann Le Floc'H, Winston Tan, Ravindra S. Telang, Srdjan M. Vlajkovic, Alfred Nuttall, William Rooney, Beau Pontré, Peter R. Thorne

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purpose To quantify spatial and temporal inflammation-induced changes in vascular permeability and macrophage infiltration in guinea-pig (GP) cochlea using MRI. Materials and Methods GPs were injected with lipopolysaccharide (LPS) to induce cochlear inflammation. One group was injected with a gadolinium based contrast agent (GBCA) and dynamic contrast enhanced (DCE)-MRI was performed at 4, 7, and 10 days after LPS treatment. A two-compartment pharmacokinetic model was used to determine the apparent rate constant of GBCA extravasation (K trans). A second group was injected with ultrasmall superparamagnetic iron oxide particles (USPIOs) and studied at 2, 3, and 7 days after LPS treatment to detect tissue USPIO uptake and correlate with histology. For both groups, control GPs were scanned similarly. Results The signal enhancement increased substantially and more rapidly at day 4 in LPS-treated than in control cochlea shortly following GBCA injection. Ktrans of LPS-treated cochlea was maximum on day 4 at 0.0218 ± 0.0032 min-1 and then decreased to control level at 0.0036 ± 0.0004 min-1 by day 10. In the second group, the relative signal intensity and T2 in cochlear perilymphatic spaces on day 2 decreased, on average, by 54% and 45%, respectively, compared with baseline and then remained under control levels by day 7. This suggests the infiltration of inflammatory cells, although unconfirmed by histology. Conclusion This provides the first measurement of cochlear vascular permeability using MRI and a quantitative evaluation of the development of cochlear inflammation. MRI holds considerable potential for the assessment of disease processes such as clinical diagnosis of conditions such as labyrinthitis. J. Magn. Reson. Imaging 2014;39:150-161. © 2013 Wiley Periodicals, Inc.

Original languageEnglish (US)
Pages (from-to)150-161
Number of pages12
JournalJournal of Magnetic Resonance Imaging
Volume39
Issue number1
DOIs
StatePublished - Jan 2014

Fingerprint

Cochlea
Inflammation
Lipopolysaccharides
Gadolinium
Contrast Media
Capillary Permeability
Histology
Labyrinthitis
Process Assessment (Health Care)
Guinea Pigs
Pharmacokinetics
Macrophages
Control Groups
Injections
Therapeutics

Keywords

  • blood-labyrinth barrier
  • cochlear inflammation
  • lipopolysaccharide
  • MRI
  • ultrasmall superparamagnetic iron oxide
  • vascular permeability

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Floc'H, J. L., Tan, W., Telang, R. S., Vlajkovic, S. M., Nuttall, A., Rooney, W., ... Thorne, P. R. (2014). Markers of cochlear inflammation using MRI. Journal of Magnetic Resonance Imaging, 39(1), 150-161. https://doi.org/10.1002/jmri.24144

Markers of cochlear inflammation using MRI. / Floc'H, Johann Le; Tan, Winston; Telang, Ravindra S.; Vlajkovic, Srdjan M.; Nuttall, Alfred; Rooney, William; Pontré, Beau; Thorne, Peter R.

In: Journal of Magnetic Resonance Imaging, Vol. 39, No. 1, 01.2014, p. 150-161.

Research output: Contribution to journalArticle

Floc'H, JL, Tan, W, Telang, RS, Vlajkovic, SM, Nuttall, A, Rooney, W, Pontré, B & Thorne, PR 2014, 'Markers of cochlear inflammation using MRI', Journal of Magnetic Resonance Imaging, vol. 39, no. 1, pp. 150-161. https://doi.org/10.1002/jmri.24144
Floc'H, Johann Le ; Tan, Winston ; Telang, Ravindra S. ; Vlajkovic, Srdjan M. ; Nuttall, Alfred ; Rooney, William ; Pontré, Beau ; Thorne, Peter R. / Markers of cochlear inflammation using MRI. In: Journal of Magnetic Resonance Imaging. 2014 ; Vol. 39, No. 1. pp. 150-161.
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