Mapping of the extracellular binding regions of the human interleukin-8 type B receptor

James A. Katancik, Ashu Sharma, Stephen J. Radel, Ernesto De Nardin

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

This study was undertaken to define the regions of the human interleukin-8 type B receptor (IL8RB) which are critical for binding the ligands interleukin-8, NAP-2 and GROα. Peptides corresponding to the N-terminus region and the first extracellular loop of the receptor demonstrated statistically significant (p = 0.001) inhibition of IL-8 control binding levels (inhibition levels of 73.0 ± 5.1% and 89.9 ± 2.2% respectively). In contrast, NAP-2 binding was inhibited only by the peptide representing the first extracellular loop (63.2 ± 2.3%), while GROα binding was inhibited by portions of the N-terminus (49.7 ± 14.9% and 41.8 ± 14.9), but not the first extracellular loop. We suggest that: a) the chemokine receptor IL8RB, known to bind three related ligands with high affinity, seems to do so via distinct contact points and b.) the first extracellular loop is significant in the binding event.

Original languageEnglish (US)
Pages (from-to)663-668
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume232
Issue number3
DOIs
StatePublished - Mar 27 1997

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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