Mapping of a putative genetic locus determining ethanol intake in the mouse

D. Goldman, R. G. Lister, John Jr Crabbe

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In the mouse, there is evidence that a single genetic locus is a major determinant of differences in ethanol intake between some preferring and non-preferring inbred strains. In this report, we present evidence from two independent experiments indicating that this locus maps to chromosome 1 and that its expressed product is the abundant protein LTW-4 (a 28 kDa, pI 5.6 protein expressed in brain, liver and kidney). The genetic association was found using a panel of 14 polypeptides of mouse brain which were visualized by two-dimensional electrophoresis and which exhibit genetic variation in isoelectric point. Fifteen BXD recombinant inbred strains and the two parental strains were typed for these loci and also tested for ethanol acceptance. Strains exhibiting the basic allele showed significantly higher ethanol acceptance. When 19 distantly related inbred mouse strains were tested for ethanol acceptance and typed for LTW-4, it was again found that strains exhibiting the basic allele showed significantly higher ethanol acceptance.

Original languageEnglish (US)
Pages (from-to)220-226
Number of pages7
JournalBrain Research
Volume420
Issue number2
DOIs
StatePublished - Sep 15 1987

Fingerprint

Genetic Loci
Ethanol
Alleles
Inbred Strains Mice
Chromosomes, Human, Pair 1
Isoelectric Point
Brain
Electrophoresis
Proteins
Kidney
Peptides
Liver

Keywords

  • Alcohol
  • Ethanol acceptance
  • Inbred strain
  • LTW-4
  • Mouse
  • Pharmacogenetics
  • Protein polymorphism
  • Recombinant inbred strain
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Mapping of a putative genetic locus determining ethanol intake in the mouse. / Goldman, D.; Lister, R. G.; Crabbe, John Jr.

In: Brain Research, Vol. 420, No. 2, 15.09.1987, p. 220-226.

Research output: Contribution to journalArticle

Goldman, D. ; Lister, R. G. ; Crabbe, John Jr. / Mapping of a putative genetic locus determining ethanol intake in the mouse. In: Brain Research. 1987 ; Vol. 420, No. 2. pp. 220-226.
@article{63c809bfdf6f42b3b86208399f2f9a43,
title = "Mapping of a putative genetic locus determining ethanol intake in the mouse",
abstract = "In the mouse, there is evidence that a single genetic locus is a major determinant of differences in ethanol intake between some preferring and non-preferring inbred strains. In this report, we present evidence from two independent experiments indicating that this locus maps to chromosome 1 and that its expressed product is the abundant protein LTW-4 (a 28 kDa, pI 5.6 protein expressed in brain, liver and kidney). The genetic association was found using a panel of 14 polypeptides of mouse brain which were visualized by two-dimensional electrophoresis and which exhibit genetic variation in isoelectric point. Fifteen BXD recombinant inbred strains and the two parental strains were typed for these loci and also tested for ethanol acceptance. Strains exhibiting the basic allele showed significantly higher ethanol acceptance. When 19 distantly related inbred mouse strains were tested for ethanol acceptance and typed for LTW-4, it was again found that strains exhibiting the basic allele showed significantly higher ethanol acceptance.",
keywords = "Alcohol, Ethanol acceptance, Inbred strain, LTW-4, Mouse, Pharmacogenetics, Protein polymorphism, Recombinant inbred strain, Two-dimensional electrophoresis",
author = "D. Goldman and Lister, {R. G.} and Crabbe, {John Jr}",
year = "1987",
month = "9",
day = "15",
doi = "10.1016/0006-8993(87)91241-8",
language = "English (US)",
volume = "420",
pages = "220--226",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Mapping of a putative genetic locus determining ethanol intake in the mouse

AU - Goldman, D.

AU - Lister, R. G.

AU - Crabbe, John Jr

PY - 1987/9/15

Y1 - 1987/9/15

N2 - In the mouse, there is evidence that a single genetic locus is a major determinant of differences in ethanol intake between some preferring and non-preferring inbred strains. In this report, we present evidence from two independent experiments indicating that this locus maps to chromosome 1 and that its expressed product is the abundant protein LTW-4 (a 28 kDa, pI 5.6 protein expressed in brain, liver and kidney). The genetic association was found using a panel of 14 polypeptides of mouse brain which were visualized by two-dimensional electrophoresis and which exhibit genetic variation in isoelectric point. Fifteen BXD recombinant inbred strains and the two parental strains were typed for these loci and also tested for ethanol acceptance. Strains exhibiting the basic allele showed significantly higher ethanol acceptance. When 19 distantly related inbred mouse strains were tested for ethanol acceptance and typed for LTW-4, it was again found that strains exhibiting the basic allele showed significantly higher ethanol acceptance.

AB - In the mouse, there is evidence that a single genetic locus is a major determinant of differences in ethanol intake between some preferring and non-preferring inbred strains. In this report, we present evidence from two independent experiments indicating that this locus maps to chromosome 1 and that its expressed product is the abundant protein LTW-4 (a 28 kDa, pI 5.6 protein expressed in brain, liver and kidney). The genetic association was found using a panel of 14 polypeptides of mouse brain which were visualized by two-dimensional electrophoresis and which exhibit genetic variation in isoelectric point. Fifteen BXD recombinant inbred strains and the two parental strains were typed for these loci and also tested for ethanol acceptance. Strains exhibiting the basic allele showed significantly higher ethanol acceptance. When 19 distantly related inbred mouse strains were tested for ethanol acceptance and typed for LTW-4, it was again found that strains exhibiting the basic allele showed significantly higher ethanol acceptance.

KW - Alcohol

KW - Ethanol acceptance

KW - Inbred strain

KW - LTW-4

KW - Mouse

KW - Pharmacogenetics

KW - Protein polymorphism

KW - Recombinant inbred strain

KW - Two-dimensional electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=0023230260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023230260&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(87)91241-8

DO - 10.1016/0006-8993(87)91241-8

M3 - Article

C2 - 3676756

AN - SCOPUS:0023230260

VL - 420

SP - 220

EP - 226

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -