MAP2 is localized to the dendrites of hippocampal neurons which develop in culture

Alfredo Caceres, Gary Banker, Oswald Steward, Lester Binder, Michael Payne

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Abstract

The distribution of the microtubule-associated protein MAP2 in cultured hippocampal neurons was studied using immunocytochemistry with monoclonal antibodies. MAP2 was preferentially localized to dendritic, but not axonal, processes even in single isolated cells which developed without making intercellular contacts. Hence regional differences in the molecular composition of the neuronal cytoskeleton can develop independently of cell interactions. The presence of MAP2 may be a useful marker for identifying dendrites in cell culture.

Original languageEnglish (US)
Pages (from-to)314-318
Number of pages5
JournalDevelopmental Brain Research
Volume13
Issue number2
DOIs
StatePublished - Apr 1984

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Keywords

  • cytoskeleton
  • dendrites
  • hippocampal neurons
  • immunocytochemistry
  • microtubule-associated proteins
  • monoclonal antibodies
  • neuronal development
  • tissue culture

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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