Map location of the pcbA mutation and physiology of the mutant

S. K. Bryan, R. E. Moses

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Many temperature-resistant revertants of a polA1 polB polCts (HS432) strain are polI+ (by either suppression of the polA1 amber allele or intragenic reversion) but remain polCts (contain a temperature-sensitive DNA polymerase III). It appears that DNA replication in such temperature-resistant revertants depends on an extragenic mutation, pcbA, already present in the parent strain and not linked to any of the DNA polymerase loci. This allele allows DNA replication dependent on DNA polymerase I and bypasses a temperature-sensitive DNA polymerase III (polC bypass), so that reversion to Po1I+ makes the strain temperature resistant. This pathway of DNA replication also supports phage and plasmid DNA replication. At restrictive temperature, these mutants display a normal response to UV irradiation but show increased sensitivity to alkylating agent methyl methanesulfonate. We have located pcbA linked to dnaA.

Original languageEnglish (US)
Pages (from-to)216-221
Number of pages6
JournalJournal of bacteriology
Volume158
Issue number1
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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