Manipulation of microspheres and biological cells with multiple agile VCSEL traps

Bing Shao, Sanja Zlatanovic, Mihrimah Ozkan, Aaron L. Birkbeck, Sadik Esener

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A microscope-integrated vertical cavity surface emitting laser (VCSEL) array trapping system capable of independent control, rotation, and batch processing of biological cells is developed and demonstrated. The trapping system is applied for manipulation of yeast cells and PC-12 cells. In our trapping system, a single VCSEL trap serves as a collector and distributor, while an array of VCSEL traps functions like a chip, enabling parallel processing of multiple objects. The trapping system here differs from earlier VCSEL tweezers set-ups in several ways, including (1) the optical traps are mobile with the addition of a static sample holder; and (2) both a single and an array of optical traps can be controlled independently by tilting mirrors at the conjugate planes of the objective back aperture, and their relative depth can be adjusted without losing trapping power. These enhancements provide an advantage for lab-on-a-chip devices that contain microfluidics, since the background flow resulting from moving the sample plane introduces complexity and uncertainty in the velocity and force analysis on microparticles or cells. In addition, independent control of traps can offer more flexibility in multi-target manipulation. Here, we address optical design considerations for keeping stable trapping performance while multiplexing. Potential improvements based on two independently controlled VCSEL arrays are discussed and related applications are investigated.

Original languageEnglish (US)
Pages (from-to)866-874
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume113
Issue number2
DOIs
StatePublished - Feb 27 2006
Externally publishedYes

Fingerprint

Surface emitting lasers
surface emitting lasers
Microspheres
manipulators
trapping
traps
cavities
cells
laser arrays
Lab-on-a-chip
batch processing
lab-on-a-chip devices
Optical design
distributors
Multiplexing
Microfluidics
Yeast
yeast
microparticles
holders

Keywords

  • Biological cells
  • Lab-on-a-chip
  • Microfluidic system
  • Micromanipulator
  • Optical tweezers
  • Vertical cavity surface emitting lasers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

Cite this

Manipulation of microspheres and biological cells with multiple agile VCSEL traps. / Shao, Bing; Zlatanovic, Sanja; Ozkan, Mihrimah; Birkbeck, Aaron L.; Esener, Sadik.

In: Sensors and Actuators, B: Chemical, Vol. 113, No. 2, 27.02.2006, p. 866-874.

Research output: Contribution to journalArticle

Shao, Bing ; Zlatanovic, Sanja ; Ozkan, Mihrimah ; Birkbeck, Aaron L. ; Esener, Sadik. / Manipulation of microspheres and biological cells with multiple agile VCSEL traps. In: Sensors and Actuators, B: Chemical. 2006 ; Vol. 113, No. 2. pp. 866-874.
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