Mammary collective cell migration involves transient loss of epithelial features and individual cell migration within the epithelium

Andrew J. Ewald, Robert J. Huebner, Hildur Palsdottir, Jessie K. Lee, Melissa J. Perez, Danielle Jorgens, Andrew N. Tauscher, Kevin J. Cheung, Zena Werb, Manfred Auer

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Normal mammary morphogenesis involves transitions between simple and multilayered epithelial organizations. We used electron microscopy and molecular markers to determine whether intercellular junctions and apico-basal polarity were maintained in the multilayered epithelium. We found that multilayered elongating ducts had polarized apical and basal tissue surfaces both in threedimensional culture and in vivo. However, individual cells were only polarized on surfaces in contact with the lumen or extracellular matrix. The basolateral marker scribble and the apical marker atypical protein kinase C zeta localized to all interior cell membranes, whereas PAR3 displayed a cytoplasmic localization, suggesting that the apico-basal polarity was incomplete. Despite membrane localization of E-cadherin and b-catenin, we did not observe a defined zonula adherens connecting interior cells. Instead, interior cells were connected through desmosomes and exhibited complex interdigitating membrane protrusions. Single-cell labeling revealed that individual cells were both protrusive and migratory within the epithelial multilayer. Inhibition of Rho kinase (ROCK) further reduced intercellular adhesion on apical and lateral surfaces but did not disrupt basal tissue organization. Following morphogenesis, segregated membrane domains were re-established and junctional complexes re-formed. We observed similar epithelial organization during mammary morphogenesis in organotypic culture and in vivo. We conclude that mammary epithelial morphogenesis involves a reversible, spatially limited, reduction in polarity and intercellular junctions and active individualistic cell migration. Our data suggest that reductions in polarity and adhesion during breast cancer progression might reflect partial recapitulation of a normal developmental program.

Original languageEnglish (US)
Pages (from-to)2638-2654
Number of pages17
JournalJournal of Cell Science
Volume125
Issue number11
DOIs
StatePublished - Jun 1 2012
Externally publishedYes

Fingerprint

Cell Movement
Breast
Morphogenesis
Epithelium
Intercellular Junctions
Membranes
Adherens Junctions
Catenins
rho-Associated Kinases
Desmosomes
Cadherins
Extracellular Matrix
Electron Microscopy
Cell Membrane
Breast Neoplasms

Keywords

  • Apico-basal polarity
  • Breast cancer
  • Epithelial morphogenesis
  • Mammary gland

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ewald, A. J., Huebner, R. J., Palsdottir, H., Lee, J. K., Perez, M. J., Jorgens, D., ... Auer, M. (2012). Mammary collective cell migration involves transient loss of epithelial features and individual cell migration within the epithelium. Journal of Cell Science, 125(11), 2638-2654. https://doi.org/10.1242/jcs.096875

Mammary collective cell migration involves transient loss of epithelial features and individual cell migration within the epithelium. / Ewald, Andrew J.; Huebner, Robert J.; Palsdottir, Hildur; Lee, Jessie K.; Perez, Melissa J.; Jorgens, Danielle; Tauscher, Andrew N.; Cheung, Kevin J.; Werb, Zena; Auer, Manfred.

In: Journal of Cell Science, Vol. 125, No. 11, 01.06.2012, p. 2638-2654.

Research output: Contribution to journalArticle

Ewald, AJ, Huebner, RJ, Palsdottir, H, Lee, JK, Perez, MJ, Jorgens, D, Tauscher, AN, Cheung, KJ, Werb, Z & Auer, M 2012, 'Mammary collective cell migration involves transient loss of epithelial features and individual cell migration within the epithelium', Journal of Cell Science, vol. 125, no. 11, pp. 2638-2654. https://doi.org/10.1242/jcs.096875
Ewald, Andrew J. ; Huebner, Robert J. ; Palsdottir, Hildur ; Lee, Jessie K. ; Perez, Melissa J. ; Jorgens, Danielle ; Tauscher, Andrew N. ; Cheung, Kevin J. ; Werb, Zena ; Auer, Manfred. / Mammary collective cell migration involves transient loss of epithelial features and individual cell migration within the epithelium. In: Journal of Cell Science. 2012 ; Vol. 125, No. 11. pp. 2638-2654.
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