Abstract
Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 327-336 |
| Number of pages | 10 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 261 |
| State | Published - 2004 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Mammalian two-hybrid assay for detecting protein-protein interactions in vivo. / Lee, Jae; Lee, Soo-Kyung.
In: Methods in molecular biology (Clifton, N.J.), Vol. 261, 2004, p. 327-336.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.
AU - Lee, Jae
AU - Lee, Soo-Kyung
PY - 2004
Y1 - 2004
N2 - Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.
AB - Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.
UR - http://www.scopus.com/inward/record.url?scp=3242671815&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3242671815&partnerID=8YFLogxK
M3 - Article
C2 - 15064467
AN - SCOPUS:3242671815
VL - 261
SP - 327
EP - 336
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -