Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.

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18 Citations (Scopus)

Abstract

Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.

Original languageEnglish (US)
Pages (from-to)327-336
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume261
StatePublished - 2004
Externally publishedYes

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Two-Hybrid System Techniques
Yeasts
Proteins
Post Translational Protein Processing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

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title = "Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.",
abstract = "Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.",
author = "Jae Lee and Soo-Kyung Lee",
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