Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde

Priscilla H. Fernandes, Manorama Kanuri, Lubomir V. Nechev, Thomas M. Harris, R. Stephen Lloyd

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site-specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5-6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals.

Original languageEnglish (US)
Pages (from-to)455-459
Number of pages5
JournalEnvironmental and Molecular Mutagenesis
Volume45
Issue number5
DOIs
StatePublished - Jun 2005

Keywords

  • Crotonaldehyde
  • Mutogenicity
  • Vinyl chloride
  • α-Me-γ-HOPdG
  • β-HOEdG

ASJC Scopus subject areas

  • Epidemiology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

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