Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde

Priscilla H. Fernandes, Manorama Kanuri, Lubomir V. Nechev, Thomas M. Harris, R. Stephen Lloyd

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site-specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5-6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals.

Original languageEnglish (US)
Pages (from-to)455-459
Number of pages5
JournalEnvironmental and Molecular Mutagenesis
Volume45
Issue number5
DOIs
StatePublished - Jun 1 2005

Keywords

  • Crotonaldehyde
  • Mutogenicity
  • Vinyl chloride
  • α-Me-γ-HOPdG
  • β-HOEdG

ASJC Scopus subject areas

  • Epidemiology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde'. Together they form a unique fingerprint.

  • Cite this