Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde

Priscilla H. Fernandes, Manorama Kanuri, Lubomir V. Nechev, Thomas M. Harris, Robert (Stephen) Lloyd

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site-specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5-6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals.

Original languageEnglish (US)
Pages (from-to)455-459
Number of pages5
JournalEnvironmental and Molecular Mutagenesis
Volume45
Issue number5
DOIs
StatePublished - Jun 2005

Fingerprint

2-butenal
Vinyl Chloride
Mutagenesis
DNA Adducts
Deoxyguanosine
Cells
chloride
DNA
Genetic Vectors
genotoxicity
Mutagens
carcinogen
lesion
Carcinogens
mutation
Mutation

Keywords

  • α-Me-γ-HOPdG
  • β-HOEdG
  • Crotonaldehyde
  • Mutogenicity
  • Vinyl chloride

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Genetics
  • Genetics(clinical)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde. / Fernandes, Priscilla H.; Kanuri, Manorama; Nechev, Lubomir V.; Harris, Thomas M.; Lloyd, Robert (Stephen).

In: Environmental and Molecular Mutagenesis, Vol. 45, No. 5, 06.2005, p. 455-459.

Research output: Contribution to journalArticle

Fernandes, Priscilla H. ; Kanuri, Manorama ; Nechev, Lubomir V. ; Harris, Thomas M. ; Lloyd, Robert (Stephen). / Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde. In: Environmental and Molecular Mutagenesis. 2005 ; Vol. 45, No. 5. pp. 455-459.
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