Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde

Priscilla H. Fernandes; Manorama Kanuri; Lubomir V. Nechev; Thomas M. Harris; R. Stephen Lloyd

doi:10.1002/em.20117

Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde

Priscilla H. Fernandes, Manorama Kanuri, Lubomir V. Nechev, Thomas M. Harris, R. Stephen Lloyd

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site-specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5-6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals.

Original language	English (US)
Pages (from-to)	455-459
Number of pages	5
Journal	Environmental and Molecular Mutagenesis
Volume	45
Issue number	5
DOIs	https://doi.org/10.1002/em.20117
State	Published - Jun 2005

Keywords

Crotonaldehyde
Mutogenicity
Vinyl chloride
α-Me-γ-HOPdG
β-HOEdG

ASJC Scopus subject areas

Epidemiology
Genetics(clinical)
Health, Toxicology and Mutagenesis

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10.1002/em.20117

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title = "Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde",

abstract = "Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site-specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5-6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals.",

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AU - Fernandes, Priscilla H.

AU - Kanuri, Manorama

AU - Nechev, Lubomir V.

AU - Harris, Thomas M.

AU - Lloyd, R. Stephen

PY - 2005/6

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AB - Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site-specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5-6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals.

KW - Crotonaldehyde

KW - Mutogenicity

KW - Vinyl chloride

KW - α-Me-γ-HOPdG

KW - β-HOEdG

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Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde

Abstract

Keywords

ASJC Scopus subject areas

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