Maleylacetoacetate isomerase (MAAI/GSTZ)-deficient mice reveal a glutathione-dependent nonenzymatic bypass in tyrosine catabolism

José Manuel Fernández-Cañón, Manfred W. Baetscher, Milton Finegold, Terry Burlingame, K. Michael Gibson, Markus Grompe

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Abstract

In mammals, the catabolic pathway of phenylalanine and tyrosine is found in liver (hepatocytes) and kidney (proximal tubular cells). There are well-described human diseases associated with deficiencies of all enzymes in this pathway except for maleylacetoacetate isomerase (MAAI), which converts maleylacetoacetate (MAA) to fumarylacetoacetate (FAA). MAAI is also known as glutathione transferase zeta (GSTZ1). Here, we describe the phenotype of mice with a targeted deletion of the MAAI (GSTZ1) gene. MAAI-deficient mice accumulated FAA and succinylacetone in urine but appeared otherwise healthy. This observation suggested that either accumulating MAA is not toxic or an alternate pathway for MAA metabolism exists. A complete redundancy of MAAI could be ruled out because substrate overload of the tyrosine catabolic pathway (administration of homogentisic acid, phenylalanine, or tyrosine) resulted in renal and hepatic damage. However, evidence for a partial bypass of MAAI activity was also found. Mice doubly mutant for MAAI and fumarylacetoacetate hydrolase (FAH) died rapidly on a normal diet, indicating that MAA could be isomerized to FAA in the absence of MAAI. Double mutants showed predominant renal injury, indicating that this organ is the primary target for the accumulated compound(s) resulting from MAAI deficiency. A glutathione-mediated isomerization of MAA to FAA independent of MAAI enzyme was demonstrated in vitro. This nonenzymatic bypass is likely responsible for the lack of a phenotype in nonstressed MAAI mutant mice.

Original languageEnglish (US)
Pages (from-to)4943-4951
Number of pages9
JournalMolecular and Cellular Biology
Volume22
Issue number13
DOIs
StatePublished - 2002
Externally publishedYes

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maleylacetoacetate isomerase
Glutathione
Tyrosine
Phenylalanine
Kidney
Homogentisic Acid
Phenotype
Poisons
Liver

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Maleylacetoacetate isomerase (MAAI/GSTZ)-deficient mice reveal a glutathione-dependent nonenzymatic bypass in tyrosine catabolism. / Fernández-Cañón, José Manuel; Baetscher, Manfred W.; Finegold, Milton; Burlingame, Terry; Gibson, K. Michael; Grompe, Markus.

In: Molecular and Cellular Biology, Vol. 22, No. 13, 2002, p. 4943-4951.

Research output: Contribution to journalArticle

Fernández-Cañón, José Manuel ; Baetscher, Manfred W. ; Finegold, Milton ; Burlingame, Terry ; Gibson, K. Michael ; Grompe, Markus. / Maleylacetoacetate isomerase (MAAI/GSTZ)-deficient mice reveal a glutathione-dependent nonenzymatic bypass in tyrosine catabolism. In: Molecular and Cellular Biology. 2002 ; Vol. 22, No. 13. pp. 4943-4951.
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