Major structural alterations of the c-sis gene are not observed in a series of tumors of the human central nervous system

Richard D. Press, Anita Misra, David Samols, David A. Goldthwait, Timothy B. Mapstone

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Expression of the c-sis oncogene, the gene encoding the B chain of platelet-derived growth factor (PDGF), may be related to initiation and/or progression of glial cell tumorigenesis by PDGF-mediated autocrine growth stimulation. As the mechanism for activation of expression of the c-sis gene in gliomas is not known, we searched for possible structural alterations of c-sis DNA in these tumors. Genomic Southern blots of DNA from 7 different cultured human glioblastoma cell lines and 15 different solid human brain tumors revealed no significant change in either the gross structure or the copy number of the c-sis gene in tumor cells vs. control cells. Activation of glioma c-sis gene expression is therefore not the result of a gross rearrangement or amplification of the c-sis gene. Expression of c-sis mRNA was detected in all of 12 different solid human brain tumors, 11 of which were of glial cell origin. However, in tissue adjacent to 5 different tumors, approximately the same level of c-sis mRNA was seen.

Original languageEnglish (US)
Pages (from-to)345-356
Number of pages12
JournalJournal of Neuro-Oncology
Volume7
Issue number4
DOIs
StatePublished - Dec 1 1989

Keywords

  • c-sis
  • glioma
  • oncogene

ASJC Scopus subject areas

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

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