Major structural alterations of the c-sis gene are not observed in a series of tumors of the human central nervous system

Richard Press, Anita Misra, David Samols, David A. Goldthwait, Timothy B. Mapstone

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Expression of the c-sis oncogene, the gene encoding the B chain of platelet-derived growth factor (PDGF), may be related to initiation and/or progression of glial cell tumorigenesis by PDGF-mediated autocrine growth stimulation. As the mechanism for activation of expression of the c-sis gene in gliomas is not known, we searched for possible structural alterations of c-sis DNA in these tumors. Genomic Southern blots of DNA from 7 different cultured human glioblastoma cell lines and 15 different solid human brain tumors revealed no significant change in either the gross structure or the copy number of the c-sis gene in tumor cells vs. control cells. Activation of glioma c-sis gene expression is therefore not the result of a gross rearrangement or amplification of the c-sis gene. Expression of c-sis mRNA was detected in all of 12 different solid human brain tumors, 11 of which were of glial cell origin. However, in tissue adjacent to 5 different tumors, approximately the same level of c-sis mRNA was seen.

Original languageEnglish (US)
Pages (from-to)345-356
Number of pages12
JournalJournal of Neuro-Oncology
Volume7
Issue number4
DOIs
StatePublished - Dec 1989
Externally publishedYes

Fingerprint

sis Genes
Central Nervous System Neoplasms
Brain Neoplasms
Glioma
Neuroglia
Proto-Oncogene Proteins c-sis
Neoplasms
Messenger RNA
DNA
Platelet-Derived Growth Factor
Glioblastoma
Southern Blotting
Carcinogenesis
Gene Expression
Cell Line
Growth

Keywords

  • c-sis
  • glioma
  • oncogene

ASJC Scopus subject areas

  • Neuroscience(all)
  • Oncology
  • Clinical Neurology
  • Cancer Research

Cite this

Major structural alterations of the c-sis gene are not observed in a series of tumors of the human central nervous system. / Press, Richard; Misra, Anita; Samols, David; Goldthwait, David A.; Mapstone, Timothy B.

In: Journal of Neuro-Oncology, Vol. 7, No. 4, 12.1989, p. 345-356.

Research output: Contribution to journalArticle

Press, Richard ; Misra, Anita ; Samols, David ; Goldthwait, David A. ; Mapstone, Timothy B. / Major structural alterations of the c-sis gene are not observed in a series of tumors of the human central nervous system. In: Journal of Neuro-Oncology. 1989 ; Vol. 7, No. 4. pp. 345-356.
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