Magnetic susceptibility shift selected imaging (MESSI) and localized 1H2O spectroscopy in living plant tissues

Kai Zhong, Xin Li, Yair Shachar-Hill, Francis Picart, Arnold Wishnia, Charles Jr Springer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Maize root segments permeated with aqueous solutions of the paramagnetic agents GdDTPA2- or DyDTPA-BMA display two well-resolved NMR peaks corresponding to the signals from intracellular and extracellular 1H2O, which arise from well-understood bulk magnetic susceptibility effects. This allows each component to be studied separately. Images obtained at each frequency with MESSI editing, and single- and multiple-voxel ('spectroscopic imaging') localized spectra, clearly indicate that the agents permeate into the interstitial spaces, and into the longitudinal (xylem/phloem) channels in the stele (core) of the root, confirming earlier assessments. We believe these are the first images of a multicellular tissue acquired in vivo exclusively from the intracellular water proton resonance. This method can be further exploited to study water transport in similar systems. Copyright (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish (US)
Pages (from-to)392-397
Number of pages6
JournalNMR in Biomedicine
Volume13
Issue number7
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Magnetic susceptibility
Spectrum Analysis
Spectroscopy
Tissue
Phloem
Imaging techniques
Xylem
Water
Zea mays
Protons
Nuclear magnetic resonance

Keywords

  • Maize
  • MRI
  • Susceptibility agent imaging
  • Transcytolemmal water transport

ASJC Scopus subject areas

  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Biophysics

Cite this

Magnetic susceptibility shift selected imaging (MESSI) and localized 1H2O spectroscopy in living plant tissues. / Zhong, Kai; Li, Xin; Shachar-Hill, Yair; Picart, Francis; Wishnia, Arnold; Springer, Charles Jr.

In: NMR in Biomedicine, Vol. 13, No. 7, 2000, p. 392-397.

Research output: Contribution to journalArticle

Zhong, Kai ; Li, Xin ; Shachar-Hill, Yair ; Picart, Francis ; Wishnia, Arnold ; Springer, Charles Jr. / Magnetic susceptibility shift selected imaging (MESSI) and localized 1H2O spectroscopy in living plant tissues. In: NMR in Biomedicine. 2000 ; Vol. 13, No. 7. pp. 392-397.
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