Magnetic resonance-coupled fluorescence tomography scanner for molecular imaging of tissue

Scott C. Davis, Brian W. Pogue, Roger Springett, Christoph Leussler, Peter Mazurkewitz, Stephen B. Tuttle, Summer Gibbs, Shudong S. Jiang, Hamid Dehghani, Keith D. Paulsen

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

A multichannel spectrally resolved optical tomography system to image molecular targets in small animals from within a clinical MRI is described. Long source/detector fibers operate in contact mode and couple light from the tissue surface in the magnet bore to 16 spectrometers, each containing two optical gratings optimized for the near infrared wavelength range. High sensitivity, cooled charge coupled devices connected to each spectrograph provide detection of the spectrally resolved signal, with exposure times that are automated for acquisition at each fiber. The design allows spectral fitting of the remission light, thereby separating the fluorescence signal from the nonspecific background, which improves the accuracy and sensitivity when imaging low fluorophore concentrations. Images of fluorescence yield are recovered using a nonlinear reconstruction approach based on the diffusion approximation of photon propagation in tissue. The tissue morphology derived from the MR images serves as an imaging template to guide the optical reconstruction algorithm. Sensitivity studies show that recovered values of indocyanine green fluorescence yield are linear to concentrations of 1 nM in a 70 mm diameter homogeneous phantom, and detection is feasible to near 10 pM. Phantom data also demonstrate imaging capabilities of imperfect fluorophore uptake in tissue volumes of clinically relevant sizes. A unique rodent MR coil provides optical fiber access for simultaneous optical and MR data acquisition of small animals. A pilot murine study using an orthotopic glioma tumor model demonstrates optical-MRI imaging of an epidermal growth factor receptor targeted fluorescent probe in vivo.

Original languageEnglish (US)
Article number064302
JournalReview of Scientific Instruments
Volume79
Issue number6
DOIs
StatePublished - 2008
Externally publishedYes

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Molecular imaging
Magnetic resonance
scanners
Tomography
magnetic resonance
tomography
Fluorescence
Tissue
Imaging techniques
fluorescence
Fluorophores
Magnetic resonance imaging
animals
sensitivity
Animals
rodents
Spectrographs
fibers
Fibers
Optical tomography

ASJC Scopus subject areas

  • Instrumentation

Cite this

Davis, S. C., Pogue, B. W., Springett, R., Leussler, C., Mazurkewitz, P., Tuttle, S. B., ... Paulsen, K. D. (2008). Magnetic resonance-coupled fluorescence tomography scanner for molecular imaging of tissue. Review of Scientific Instruments, 79(6), [064302]. https://doi.org/10.1063/1.2919131

Magnetic resonance-coupled fluorescence tomography scanner for molecular imaging of tissue. / Davis, Scott C.; Pogue, Brian W.; Springett, Roger; Leussler, Christoph; Mazurkewitz, Peter; Tuttle, Stephen B.; Gibbs, Summer; Jiang, Shudong S.; Dehghani, Hamid; Paulsen, Keith D.

In: Review of Scientific Instruments, Vol. 79, No. 6, 064302, 2008.

Research output: Contribution to journalArticle

Davis, SC, Pogue, BW, Springett, R, Leussler, C, Mazurkewitz, P, Tuttle, SB, Gibbs, S, Jiang, SS, Dehghani, H & Paulsen, KD 2008, 'Magnetic resonance-coupled fluorescence tomography scanner for molecular imaging of tissue', Review of Scientific Instruments, vol. 79, no. 6, 064302. https://doi.org/10.1063/1.2919131
Davis, Scott C. ; Pogue, Brian W. ; Springett, Roger ; Leussler, Christoph ; Mazurkewitz, Peter ; Tuttle, Stephen B. ; Gibbs, Summer ; Jiang, Shudong S. ; Dehghani, Hamid ; Paulsen, Keith D. / Magnetic resonance-coupled fluorescence tomography scanner for molecular imaging of tissue. In: Review of Scientific Instruments. 2008 ; Vol. 79, No. 6.
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