Macular lutein and zeaxanthin are related to brain lutein and zeaxanthin in primates

Rohini Vishwanathan, Martha Neuringer, D. Max Snodderly, Wolfgang Schalch, Elizabeth J. Johnson

    Research output: Contribution to journalArticle

    77 Citations (Scopus)

    Abstract

    Objectives: Xanthophyll pigments lutein and zeaxanthin cross the blood-retina barrier to preferentially accumulate in the macular region of the neural retina. There they form macular pigment, protecting the retina from blue light damage and oxidative stress. Lutein and zeaxanthin also accumulate in brain tissue. The objective of the study was to evaluate the relationship between retinal and brain levels of these xanthophylls in non-human primates. Methods: Study animals included rhesus monkeys reared on diets devoid of xanthophylls that were subsequently fed pure lutein or pure zeaxanthin (both at 3.9 μmol/kg per day, n = 6/group) and normal rhesus monkeys fed a stock diet (0.26 μmol/kg per day lutein and 0.24 μmol/kg per day zeaxanthin, n = 5). Retina (4 mm macular punch, 4-8 mm annulus, and periphery) and brain tissue (cerebellum, frontal cortex, occipital cortex, and pons) from the same animals were analyzed by reverse-phase highperformance liquid chromatography. Results: Lutein in the macula and annulus was significantly related to lutein levels in the cerebellum, occipital cortex, and pons, both in bivariate analysis and after adjusting for age, sex and n-3 fatty acid status. In the frontal cortex the relationship was marginally significant. Macular zeaxanthin was significantly related to zeaxanthin in the cerebellum and frontal cortex, while the relationship was marginally significant in the occipital cortex and pons in a bivariate model. Discussion: An integrated measure of total macular pigment optical density, which can be measured noninvasively, has the potential to be used as a biomarker to assess brain lutein and zeaxanthin status.

    Original languageEnglish (US)
    Pages (from-to)21-29
    Number of pages9
    JournalNutritional Neuroscience
    Volume16
    Issue number1
    DOIs
    StatePublished - Jan 2013

    Fingerprint

    Lutein
    Primates
    Xanthophylls
    Brain
    Occipital Lobe
    Pons
    Retina
    Frontal Lobe
    Cerebellum
    Macaca mulatta
    Diet
    Omega-3 Fatty Acids
    Reverse-Phase Chromatography
    Zeaxanthins
    Oxidative Stress
    Biomarkers
    Light

    Keywords

    • Brain
    • Cognition
    • Lutein
    • Macula
    • Zeaxanthin

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Nutrition and Dietetics
    • Neuroscience(all)

    Cite this

    Macular lutein and zeaxanthin are related to brain lutein and zeaxanthin in primates. / Vishwanathan, Rohini; Neuringer, Martha; Max Snodderly, D.; Schalch, Wolfgang; Johnson, Elizabeth J.

    In: Nutritional Neuroscience, Vol. 16, No. 1, 01.2013, p. 21-29.

    Research output: Contribution to journalArticle

    Vishwanathan, Rohini ; Neuringer, Martha ; Max Snodderly, D. ; Schalch, Wolfgang ; Johnson, Elizabeth J. / Macular lutein and zeaxanthin are related to brain lutein and zeaxanthin in primates. In: Nutritional Neuroscience. 2013 ; Vol. 16, No. 1. pp. 21-29.
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    abstract = "Objectives: Xanthophyll pigments lutein and zeaxanthin cross the blood-retina barrier to preferentially accumulate in the macular region of the neural retina. There they form macular pigment, protecting the retina from blue light damage and oxidative stress. Lutein and zeaxanthin also accumulate in brain tissue. The objective of the study was to evaluate the relationship between retinal and brain levels of these xanthophylls in non-human primates. Methods: Study animals included rhesus monkeys reared on diets devoid of xanthophylls that were subsequently fed pure lutein or pure zeaxanthin (both at 3.9 μmol/kg per day, n = 6/group) and normal rhesus monkeys fed a stock diet (0.26 μmol/kg per day lutein and 0.24 μmol/kg per day zeaxanthin, n = 5). Retina (4 mm macular punch, 4-8 mm annulus, and periphery) and brain tissue (cerebellum, frontal cortex, occipital cortex, and pons) from the same animals were analyzed by reverse-phase highperformance liquid chromatography. Results: Lutein in the macula and annulus was significantly related to lutein levels in the cerebellum, occipital cortex, and pons, both in bivariate analysis and after adjusting for age, sex and n-3 fatty acid status. In the frontal cortex the relationship was marginally significant. Macular zeaxanthin was significantly related to zeaxanthin in the cerebellum and frontal cortex, while the relationship was marginally significant in the occipital cortex and pons in a bivariate model. Discussion: An integrated measure of total macular pigment optical density, which can be measured noninvasively, has the potential to be used as a biomarker to assess brain lutein and zeaxanthin status.",
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    T1 - Macular lutein and zeaxanthin are related to brain lutein and zeaxanthin in primates

    AU - Vishwanathan, Rohini

    AU - Neuringer, Martha

    AU - Max Snodderly, D.

    AU - Schalch, Wolfgang

    AU - Johnson, Elizabeth J.

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    N2 - Objectives: Xanthophyll pigments lutein and zeaxanthin cross the blood-retina barrier to preferentially accumulate in the macular region of the neural retina. There they form macular pigment, protecting the retina from blue light damage and oxidative stress. Lutein and zeaxanthin also accumulate in brain tissue. The objective of the study was to evaluate the relationship between retinal and brain levels of these xanthophylls in non-human primates. Methods: Study animals included rhesus monkeys reared on diets devoid of xanthophylls that were subsequently fed pure lutein or pure zeaxanthin (both at 3.9 μmol/kg per day, n = 6/group) and normal rhesus monkeys fed a stock diet (0.26 μmol/kg per day lutein and 0.24 μmol/kg per day zeaxanthin, n = 5). Retina (4 mm macular punch, 4-8 mm annulus, and periphery) and brain tissue (cerebellum, frontal cortex, occipital cortex, and pons) from the same animals were analyzed by reverse-phase highperformance liquid chromatography. Results: Lutein in the macula and annulus was significantly related to lutein levels in the cerebellum, occipital cortex, and pons, both in bivariate analysis and after adjusting for age, sex and n-3 fatty acid status. In the frontal cortex the relationship was marginally significant. Macular zeaxanthin was significantly related to zeaxanthin in the cerebellum and frontal cortex, while the relationship was marginally significant in the occipital cortex and pons in a bivariate model. Discussion: An integrated measure of total macular pigment optical density, which can be measured noninvasively, has the potential to be used as a biomarker to assess brain lutein and zeaxanthin status.

    AB - Objectives: Xanthophyll pigments lutein and zeaxanthin cross the blood-retina barrier to preferentially accumulate in the macular region of the neural retina. There they form macular pigment, protecting the retina from blue light damage and oxidative stress. Lutein and zeaxanthin also accumulate in brain tissue. The objective of the study was to evaluate the relationship between retinal and brain levels of these xanthophylls in non-human primates. Methods: Study animals included rhesus monkeys reared on diets devoid of xanthophylls that were subsequently fed pure lutein or pure zeaxanthin (both at 3.9 μmol/kg per day, n = 6/group) and normal rhesus monkeys fed a stock diet (0.26 μmol/kg per day lutein and 0.24 μmol/kg per day zeaxanthin, n = 5). Retina (4 mm macular punch, 4-8 mm annulus, and periphery) and brain tissue (cerebellum, frontal cortex, occipital cortex, and pons) from the same animals were analyzed by reverse-phase highperformance liquid chromatography. Results: Lutein in the macula and annulus was significantly related to lutein levels in the cerebellum, occipital cortex, and pons, both in bivariate analysis and after adjusting for age, sex and n-3 fatty acid status. In the frontal cortex the relationship was marginally significant. Macular zeaxanthin was significantly related to zeaxanthin in the cerebellum and frontal cortex, while the relationship was marginally significant in the occipital cortex and pons in a bivariate model. Discussion: An integrated measure of total macular pigment optical density, which can be measured noninvasively, has the potential to be used as a biomarker to assess brain lutein and zeaxanthin status.

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    KW - Macula

    KW - Zeaxanthin

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