Macrophage EP4 Deficiency Increases Apoptosis and Suppresses Early Atherosclerosis

Vladimir R. Babaev, Joshua D. Chew, Lei Ding, Sarah Davis, Matthew D. Breyer, Richard M. Breyer, John A. Oates, Sergio Fazio, MacRae F. Linton

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

Prostaglandin (PG) E2, a major product of activated macrophages, has been implicated in atherosclerosis and plaque rupture. The PGE2 receptors, EP2 and EP4, are expressed in atherosclerotic lesions and are known to inhibit apoptosis in cancer cells. To examine the roles of macrophage EP4 and EP2 in apoptosis and early atherosclerosis, fetal liver cell transplantation was used to generate LDLR-/- mice chimeric for EP2-/- or EP4-/- hematopoietic cells. After 8 weeks on a Western diet, EP4-/- → LDLR-/- mice, but not EP2-/- → LDLR-/- mice, had significantly reduced aortic atherosclerosis with increased apoptotic cells in the lesions. EP4-/- peritoneal macrophages had increased sensitivity to proapoptotic stimuli, including palmitic acid and free cholesterol loading, which was accompanied by suppression of activity of p-Akt, p-Bad, and NF-κB-regulated genes. Thus, EP4 deficiency inhibits the PI3K/Akt and NF-κB pathways compromising macrophage survival and suppressing early atherosclerosis, identifying macrophage EP4-signaling pathways as molecular targets for modulating the development of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)492-501
Number of pages10
JournalCell Metabolism
Volume8
Issue number6
DOIs
StatePublished - Dec 6 2008
Externally publishedYes

Keywords

  • HUMDISEASE
  • SIGNALING

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Macrophage EP4 Deficiency Increases Apoptosis and Suppresses Early Atherosclerosis'. Together they form a unique fingerprint.

Cite this