@article{01280d54a69b4d77b8999d377042271f,
title = "Macrophage-Dependent Cytoplasmic Transfer during Melanoma Invasion In Vivo",
abstract = "Interactions between tumor cells and tumor-associated macrophages play critical roles in the initiation of tumor cell motility. To capture the cellular interactions of the tumor microenvironment with high-resolution imaging, we directly visualized tumor cells and their interactions with macrophages in zebrafish. Live imaging in zebrafish revealed that macrophages are dynamic, yet maintain sustained contact with tumor cells. In addition, the recruitment of macrophages to tumor cells promotes tumor cell dissemination. Using a Cre/LoxP strategy, we found that macrophages transfer cytoplasm to tumor cells in zebrafish and mouse models. Remarkably, macrophage cytoplasmic transfer correlated with melanoma cell dissemination. We further found that macrophages transfer cytoplasm to tumor cells upon cell contact in vitro. Thus, we present a model in which macrophage/tumor cell contact allows for the transfer of cytoplasmic molecules from macrophages to tumor cells corresponding to increased tumor cell motility and dissemination. Roh-Johnson et al. show that macrophages transfer cytoplasmic molecules to tumor cells via cell contact in zebrafish and mouse. Cytoplasmic transfer correlates with increased tumor cell dissemination in vivo and altered tumor cell migration in vitro. These results uncover a mode of contact-dependent cell-to-cell communication in the tumor microenvironment.",
keywords = "cell migration, contact-dependent cell communication, cytoplasmic transfer, macrophages, melanoma, mouse, tumor microenvironment, zebrafish",
author = "Minna Roh-Johnson and Shah, {Arish N.} and Stonick, {Jason A.} and Poudel, {Kumud R.} and Julia Kargl and Yang, {Grace H.} and {di Martino}, Julie and Hernandez, {Rafael E.} and Gast, {Charles E.} and Zarour, {Luai R.} and Susumu Antoku and Houghton, {A. Mc Garry} and Bravo-Cordero, {Jose Javier} and Wong, {Melissa H.} and John Condeelis and Moens, {Cecilia B.}",
note = "Funding Information: We would like to thank Cyrus Ghajar, Julie Rytlewski, and members of the Moens lab for discussions; Richard White, David Parichy, Adam Hurlstone, David Tobin, and Susan Brockerhoff for zebrafish reagents; Jonathan Cooper, Robert Eisenman, and Mark Groudine for equipment and reagents; Nishi Jain for help with macrophage subtype quantification; Rachel Garcia for expert animal care; and the Fred Hutchinson Cancer Center Flow Cytometry Core for help with experiments. This work was funded by NIH K99CA190836 and FHCRC Cooperative Center for Excellence in Hematology Pilot and Feasibility grant (to M.R.J.), NIH T32CA080416 and AHA 14POST18230006 (to K.R.P.), NIH R37 AI054503 (to R.E.H.), Fondation ARC pour la Recherche sur le Cancer (to J.d.M.), NIH K22CA196750 and NIH P30 CA196521 (to J.J.B.-C.), NIH R01CA188341 (to A.M.H.), European Commission FP7-PEOPLE-201-IOF 331255 (to J.K.), OHSU Center for Women's Health Circle of Giving Grant and Crohn's and Colitis Foundation of America & Prospect Creek Foundation (to M.H.W.), and NIH NS082567 and NIH R21CA195126 (to C.B.M.). Funding Information: We would like to thank Cyrus Ghajar, Julie Rytlewski, and members of the Moens lab for discussions; Richard White, David Parichy, Adam Hurlstone, David Tobin, and Susan Brockerhoff for zebrafish reagents; Jonathan Cooper, Robert Eisenman, and Mark Groudine for equipment and reagents; Nishi Jain for help with macrophage subtype quantification; Rachel Garcia for expert animal care; and the Fred Hutchinson Cancer Center Flow Cytometry Core for help with experiments. This work was funded by NIH K99CA190836 and FHCRC Cooperative Center for Excellence in Hematology Pilot and Feasibility grant (to M.R.J.), NIH T32CA080416 and AHA 14POST18230006 (to K.R.P.), NIH R37 AI054503 (to R.E.H.), Fondation ARC pour la Recherche sur le Cancer (to J.d.M.), NIH K22CA196750 and NIH P30 CA196521 (to J.J.B.-C.), NIH R01CA188341 (to A.M.H.), European Commission FP7-PEOPLE-201-IOF 331255 (to J.K.), OHSU Center for Women's Health Circle of Giving Grant and Crohn's and Colitis Foundation of America & Prospect Creek Foundation (to M.H.W.), and NIH NS082567 and NIH R21CA195126 (to C.B.M.). Publisher Copyright: {\textcopyright} 2017",
year = "2017",
month = dec,
day = "4",
doi = "10.1016/j.devcel.2017.11.003",
language = "English (US)",
volume = "43",
pages = "549--562.e6",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "5",
}