Macaque blood-derived antigen-presenting cells elicit SIV-specific immune responses

Yong De Zhu, Kevin Koo, Jeffrey D. Bradshaw, William F. Sutton, La Rene Kuller, Richard Bucala, David Anderson, Sally P. Mossman, François Villinger, Nancy L. Haigwood

    Research output: Contribution to journalArticle

    17 Scopus citations

    Abstract

    Natural blood-borne antigen-presenting cells (APCs) were tested for their ability to augment antigen presentation for SIV vaccines. Fibrocytes and monocyte-derived dendritic cells (DCs) were isolated from multiple Macaca fascicularis. Macaque fibrocytes displayed the characteristic cellular morphology and stained positive for CD34 and collagen, as observed in human and murine fibrocytes. Macaque DCs were generated from monocytes by culturing in granulocyte-macrophage colony stimulating factor and interleukin-4 (IL-4). Two days after maturation, cells were enriched for the DC marker CD83. Fibrocytes and DCs were each transfected with green fluorescence protein expression plasmids or DNA expression vectors encoding all of the SIVmne structural and regulatory genes. Autologous DCs were reinfused into macaques subcutaneously (sc) following transfection; mixing with recombinant SIV antigens or inactivated whole SIV in vitro; or mock-treatment. Autologous monocyte-derived DCs pulsed with whole inactivated SIV were re-infused and elicited cellular and/or humoral responses in vivo in eight of ten vaccinated macaques. (C) Munksgaard, Copenhagen.

    Original languageEnglish (US)
    Pages (from-to)182-192
    Number of pages11
    JournalJournal of medical primatology
    Volume29
    Issue number3-4
    DOIs
    StatePublished - 2000

    Keywords

    • Dendritic cells
    • Fibrocytes
    • Primate models

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • veterinary(all)

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    Zhu, Y. D., Koo, K., Bradshaw, J. D., Sutton, W. F., Kuller, L. R., Bucala, R., Anderson, D., Mossman, S. P., Villinger, F., & Haigwood, N. L. (2000). Macaque blood-derived antigen-presenting cells elicit SIV-specific immune responses. Journal of medical primatology, 29(3-4), 182-192. https://doi.org/10.1034/j.1600-0684.2000.290312.x