Macaque blood-derived antigen-presenting cells elicit SIV-specific immune responses

Yong De Zhu; Kevin Koo; Jeffrey D. Bradshaw; William F. Sutton; La Rene Kuller; Richard Bucala; David Anderson; Sally P. Mossman; François Villinger; Nancy L. Haigwood

doi:10.1034/j.1600-0684.2000.290312.x

Macaque blood-derived antigen-presenting cells elicit SIV-specific immune responses

Yong De Zhu, Kevin Koo, Jeffrey D. Bradshaw, William F. Sutton, La Rene Kuller, Richard Bucala, David Anderson, Sally P. Mossman, François Villinger, Nancy L. Haigwood

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Natural blood-borne antigen-presenting cells (APCs) were tested for their ability to augment antigen presentation for SIV vaccines. Fibrocytes and monocyte-derived dendritic cells (DCs) were isolated from multiple Macaca fascicularis. Macaque fibrocytes displayed the characteristic cellular morphology and stained positive for CD34 and collagen, as observed in human and murine fibrocytes. Macaque DCs were generated from monocytes by culturing in granulocyte-macrophage colony stimulating factor and interleukin-4 (IL-4). Two days after maturation, cells were enriched for the DC marker CD83. Fibrocytes and DCs were each transfected with green fluorescence protein expression plasmids or DNA expression vectors encoding all of the SIVmne structural and regulatory genes. Autologous DCs were reinfused into macaques subcutaneously (sc) following transfection; mixing with recombinant SIV antigens or inactivated whole SIV in vitro; or mock-treatment. Autologous monocyte-derived DCs pulsed with whole inactivated SIV were re-infused and elicited cellular and/or humoral responses in vivo in eight of ten vaccinated macaques. (C) Munksgaard, Copenhagen.

Original language	English (US)
Pages (from-to)	182-192
Number of pages	11
Journal	Journal of medical primatology
Volume	29
Issue number	3-4
DOIs	https://doi.org/10.1034/j.1600-0684.2000.290312.x
State	Published - 2000
Externally published	Yes

Keywords

Dendritic cells
Fibrocytes
Primate models

ASJC Scopus subject areas

Animal Science and Zoology
General Veterinary

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10.1034/j.1600-0684.2000.290312.x

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title = "Macaque blood-derived antigen-presenting cells elicit SIV-specific immune responses",

abstract = "Natural blood-borne antigen-presenting cells (APCs) were tested for their ability to augment antigen presentation for SIV vaccines. Fibrocytes and monocyte-derived dendritic cells (DCs) were isolated from multiple Macaca fascicularis. Macaque fibrocytes displayed the characteristic cellular morphology and stained positive for CD34 and collagen, as observed in human and murine fibrocytes. Macaque DCs were generated from monocytes by culturing in granulocyte-macrophage colony stimulating factor and interleukin-4 (IL-4). Two days after maturation, cells were enriched for the DC marker CD83. Fibrocytes and DCs were each transfected with green fluorescence protein expression plasmids or DNA expression vectors encoding all of the SIVmne structural and regulatory genes. Autologous DCs were reinfused into macaques subcutaneously (sc) following transfection; mixing with recombinant SIV antigens or inactivated whole SIV in vitro; or mock-treatment. Autologous monocyte-derived DCs pulsed with whole inactivated SIV were re-infused and elicited cellular and/or humoral responses in vivo in eight of ten vaccinated macaques. (C) Munksgaard, Copenhagen.",

keywords = "Dendritic cells, Fibrocytes, Primate models",

author = "Zhu, {Yong De} and Kevin Koo and Bradshaw, {Jeffrey D.} and Sutton, {William F.} and Kuller, {La Rene} and Richard Bucala and David Anderson and Mossman, {Sally P.} and Fran{\c c}ois Villinger and Haigwood, {Nancy L.}",

year = "2000",

doi = "10.1034/j.1600-0684.2000.290312.x",

language = "English (US)",

volume = "29",

pages = "182--192",

journal = "Journal of medical primatology",

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T1 - Macaque blood-derived antigen-presenting cells elicit SIV-specific immune responses

AU - Zhu, Yong De

AU - Koo, Kevin

AU - Bradshaw, Jeffrey D.

AU - Sutton, William F.

AU - Kuller, La Rene

AU - Bucala, Richard

AU - Anderson, David

AU - Mossman, Sally P.

AU - Villinger, François

AU - Haigwood, Nancy L.

PY - 2000

Y1 - 2000

N2 - Natural blood-borne antigen-presenting cells (APCs) were tested for their ability to augment antigen presentation for SIV vaccines. Fibrocytes and monocyte-derived dendritic cells (DCs) were isolated from multiple Macaca fascicularis. Macaque fibrocytes displayed the characteristic cellular morphology and stained positive for CD34 and collagen, as observed in human and murine fibrocytes. Macaque DCs were generated from monocytes by culturing in granulocyte-macrophage colony stimulating factor and interleukin-4 (IL-4). Two days after maturation, cells were enriched for the DC marker CD83. Fibrocytes and DCs were each transfected with green fluorescence protein expression plasmids or DNA expression vectors encoding all of the SIVmne structural and regulatory genes. Autologous DCs were reinfused into macaques subcutaneously (sc) following transfection; mixing with recombinant SIV antigens or inactivated whole SIV in vitro; or mock-treatment. Autologous monocyte-derived DCs pulsed with whole inactivated SIV were re-infused and elicited cellular and/or humoral responses in vivo in eight of ten vaccinated macaques. (C) Munksgaard, Copenhagen.

AB - Natural blood-borne antigen-presenting cells (APCs) were tested for their ability to augment antigen presentation for SIV vaccines. Fibrocytes and monocyte-derived dendritic cells (DCs) were isolated from multiple Macaca fascicularis. Macaque fibrocytes displayed the characteristic cellular morphology and stained positive for CD34 and collagen, as observed in human and murine fibrocytes. Macaque DCs were generated from monocytes by culturing in granulocyte-macrophage colony stimulating factor and interleukin-4 (IL-4). Two days after maturation, cells were enriched for the DC marker CD83. Fibrocytes and DCs were each transfected with green fluorescence protein expression plasmids or DNA expression vectors encoding all of the SIVmne structural and regulatory genes. Autologous DCs were reinfused into macaques subcutaneously (sc) following transfection; mixing with recombinant SIV antigens or inactivated whole SIV in vitro; or mock-treatment. Autologous monocyte-derived DCs pulsed with whole inactivated SIV were re-infused and elicited cellular and/or humoral responses in vivo in eight of ten vaccinated macaques. (C) Munksgaard, Copenhagen.

KW - Dendritic cells

KW - Fibrocytes

KW - Primate models

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ER -

Macaque blood-derived antigen-presenting cells elicit SIV-specific immune responses

Abstract

Keywords

ASJC Scopus subject areas

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