Müllerian inhibiting substance levels at the time of HCG administration in IVF cycles predict both ovarian reserve and embryo morphology

T. Silberstein, D. T. MacLaughlin, I. Shai, J. R. Trimarchi, G. Lambert-Messerlian, D. B. Seifer, D. L. Keefe, A. S. Blazar

Research output: Contribution to journalArticle

149 Scopus citations

Abstract

Background: Pre-antral and early antral follicles secrete Müllerian inhibiting substance (MIS), suggesting that MIS may directly reflect ovarian reserve. Since little is known about how ovarian reserve affects oocyte quality, we attempt here to assess the predictive value of MIS on embryo morphology and IVF outcome. To do so, we measured MIS at the time of HCG administration 36 h prior to oocyte retrieval. Methods: A total of 257 patients undergoing IVF were prospectively recruited. We measured MIS levels by enzyme-linked immunosorbent assay at the time of HCG, and compared the MIS values to day 3 FSH levels in the prediction of embryo morphology and IVF outcome. Results: The distribution of MIS levels was skewed, with a median of 2.7 ng/ml (range 0 to 28.5 ng/ml). MIS values at the time of HCG administration inversely correlated with basal FSH levels (P = 0.002), and both correlated significantly with patient age, number of mature follicles, number of oocytes retrieved and serum estradiol levels. MIS levels correlated significantly with a greater number of 6-cell embryos and better embryo morphology score, while basal FSH levels did not correlate with these outcome variables. MIS levels > 2.7 ng/ml portended improved oocyte quality as reflected in a higher implantation rate (P = 0.001) and a trend toward a better clinical pregnancy rate (P = 0.084). Conclusions: MIS levels seem to predict not only ovarian reserve, but also embryo morphology. Measurement of MIS at the time of HCG administration may, therefore, in the future improve management of patients undergoing treatments with assisted reproductive technology.

Original languageEnglish (US)
Pages (from-to)159-163
Number of pages5
JournalHuman Reproduction
Volume21
Issue number1
DOIs
StatePublished - Jan 2006

Keywords

  • Embryo morphology
  • IVF
  • Müllerian inhibiting substance
  • Ovarian reserve
  • Pregnancy rate

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Fingerprint Dive into the research topics of 'Müllerian inhibiting substance levels at the time of HCG administration in IVF cycles predict both ovarian reserve and embryo morphology'. Together they form a unique fingerprint.

  • Cite this

    Silberstein, T., MacLaughlin, D. T., Shai, I., Trimarchi, J. R., Lambert-Messerlian, G., Seifer, D. B., Keefe, D. L., & Blazar, A. S. (2006). Müllerian inhibiting substance levels at the time of HCG administration in IVF cycles predict both ovarian reserve and embryo morphology. Human Reproduction, 21(1), 159-163. https://doi.org/10.1093/humrep/dei270