LytTR Regulatory Systems: A potential new class of prokaryotic sensory system

Zhengzhong Zou, Hua Qin, Amanda E. Brenner, Rahul Raghavan, Jess A. Millar, Qiang Gu, Zhoujie Xie, Jens Kreth, Justin Merritt

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The most commonly studied prokaryotic sensory signal transduction systems include the one-component systems, phosphosignaling systems, extracytoplasmic function (ECF) sigma factor systems, and the various types of second messenger systems. Recently, we described the regulatory role of two separate sensory systems in Streptococcus mutans that jointly control bacteriocin gene expression, natural competence development, as well as a cell death pathway, yet they do not function via any of the currently recognized signal transduction paradigms. These systems, which we refer to as LytTR Regulatory Systems (LRS), minimally consist of two proteins, a transcription regulator from the LytTR Family and a transmembrane protein inhibitor of this transcription regulator. Here, we provide evidence suggesting that LRS are a unique uncharacterized class of prokaryotic sensory system. LRS exist in a basal inactive state. However, when LRS membrane inhibitor proteins are inactivated, an autoregulatory positive feedback loop is triggered due to LRS regulator protein interactions with direct repeat sequences located just upstream of the -35 sequences of LRS operon promoters. Uncharacterized LRS operons are widely encoded by a vast array of Gram positive and Gram negative bacteria as well as some archaea. These operons also contain unique direct repeat sequences immediately upstream of their operon promoters indicating that positive feedback autoregulation is a globally conserved feature of LRS. Despite the surprisingly widespread occurrence of LRS operons, the only characterized examples are those of S. mutans. Therefore, the current study provides a useful roadmap to investigate LRS function in the numerous other LRS-encoding organisms.

Original languageEnglish (US)
Pages (from-to)e1007709
JournalPLoS Genetics
Volume14
Issue number10
DOIs
StatePublished - Oct 1 2018

Fingerprint

sensory system
operon
Operon
protein
Streptococcus mutans
Nucleic Acid Repetitive Sequences
inhibitor
signal transduction
Signal Transduction
transcription factors
promoter regions
sigma factors
Sigma Factor
autoregulation
Bacteriocins
regulatory sequences
Proteins
transmembrane proteins
proteins
second messengers

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

LytTR Regulatory Systems : A potential new class of prokaryotic sensory system. / Zou, Zhengzhong; Qin, Hua; Brenner, Amanda E.; Raghavan, Rahul; Millar, Jess A.; Gu, Qiang; Xie, Zhoujie; Kreth, Jens; Merritt, Justin.

In: PLoS Genetics, Vol. 14, No. 10, 01.10.2018, p. e1007709.

Research output: Contribution to journalArticle

Zou, Z, Qin, H, Brenner, AE, Raghavan, R, Millar, JA, Gu, Q, Xie, Z, Kreth, J & Merritt, J 2018, 'LytTR Regulatory Systems: A potential new class of prokaryotic sensory system', PLoS Genetics, vol. 14, no. 10, pp. e1007709. https://doi.org/10.1371/journal.pgen.1007709
Zou Z, Qin H, Brenner AE, Raghavan R, Millar JA, Gu Q et al. LytTR Regulatory Systems: A potential new class of prokaryotic sensory system. PLoS Genetics. 2018 Oct 1;14(10):e1007709. https://doi.org/10.1371/journal.pgen.1007709
Zou, Zhengzhong ; Qin, Hua ; Brenner, Amanda E. ; Raghavan, Rahul ; Millar, Jess A. ; Gu, Qiang ; Xie, Zhoujie ; Kreth, Jens ; Merritt, Justin. / LytTR Regulatory Systems : A potential new class of prokaryotic sensory system. In: PLoS Genetics. 2018 ; Vol. 14, No. 10. pp. e1007709.
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