Lysosomal killing of Mycobacterium mediated by ubiquitin-derived peptides is enhanced by autophagy

Sylvie Alonso, Kevin Pethe, David G. Russell, Georgiana E. Purdy

Research output: Contribution to journalArticle

238 Scopus citations

Abstract

Mycobacterium tuberculosis parasitizes resting macrophages yet is killed by activated macrophages through both oxidative and nonoxidative mechanisms. Nonoxidative mechanisms are linked to the maturation of the bacteria-containing phagosome into an acidified, hydrolytically active compartment. We describe here a mechanism for killing Mycobacteria in the lysosomal compartment through the activity of peptides generated by the hydrolysis of ubiquitin. The induction of autophagy in infected macrophages enhanced the delivery of ubiquitin conjugates to the lysosome and increased the bactericidal capacity of the lysosomal soluble fraction. The accumulation of ubiquitinated proteins in the autophagolysosome provides one possible mechanism behind the antimicrobial activities observed for a range of pathogens in autophagous host cells.

Original languageEnglish (US)
Pages (from-to)6031-6036
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number14
DOIs
StatePublished - Apr 3 2007

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Keywords

  • Lysosome
  • Macrophage
  • Phagosome
  • Tuberculosis

ASJC Scopus subject areas

  • General

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