Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N- terminal kinase activity

Xianjun Fang, Spencer Gibson, Michele Flowers, Tatsuro Furui, Robert C. Bast, Gordon Mills

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Lysophosphatidylcholine (lyso-PC), a natural lipid generated through the action of phospholipase A2 on membrane phosphatidylcholine, has been implicated in atherogenesis and the inflammatory process. In vitro studies have established a role for lyso-PC in modulation of gene expression and other cellular responses including differentiation and proliferation. There is also evidence that lyso-PC may act as an intracellular second messenger transducing signals elicited from membrane-associated receptors. The mechanisms behind the diverse activities of lyso-PC are poorly understood. We report, in this study, that treatment of cultured cells with exogenous lyso- PC, at nontoxic concentrations, potently induced activator protein-1 (AP-1) DNA binding and transcriptional activity independent of well known AP-1 activators, protein kinase C or mitogen-activated protein kinases ERK1 and ERK2. Lyso-PC also activated the c-Jun N-terminal kinase (JNK/SAPK), a recently characterized member of the mitogen-activated protein kinase family, known to activate AP-1. The stimulated JNK and AP-1 activities probably mediate or contribute to some bioactive effects of lyso-PC.

Original languageEnglish (US)
Pages (from-to)13683-13689
Number of pages7
JournalJournal of Biological Chemistry
Volume272
Issue number21
DOIs
StatePublished - May 23 1997
Externally publishedYes

Fingerprint

Lysophosphatidylcholines
JNK Mitogen-Activated Protein Kinases
Transcription Factor AP-1
Mitogen-Activated Protein Kinases
Membranes
Phospholipases A2
Second Messenger Systems
Phosphatidylcholines
Gene expression
Protein Kinase C
Cultured Cells
Atherosclerosis
Cells
Modulation
Lipids
Gene Expression
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N- terminal kinase activity. / Fang, Xianjun; Gibson, Spencer; Flowers, Michele; Furui, Tatsuro; Bast, Robert C.; Mills, Gordon.

In: Journal of Biological Chemistry, Vol. 272, No. 21, 23.05.1997, p. 13683-13689.

Research output: Contribution to journalArticle

Fang, Xianjun ; Gibson, Spencer ; Flowers, Michele ; Furui, Tatsuro ; Bast, Robert C. ; Mills, Gordon. / Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N- terminal kinase activity. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 21. pp. 13683-13689.
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