Lysophosphatidylcholine modulates neutrophil oxidant production through elevation of cyclic AMP

Phoebe Lin, Emily J. Welch, Xiao Pei Gao, Asrar B. Malik, Richard D. Ye

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Lysophosphatidylcholine (LPC) is an oxidized phospholipid present in micromolar concentrations in blood and inflamed tissues. The effects of LPC on neutrophil functions remain incompletely understood, because conflicting reports exist for its stimulatory and inhibitory roles. We report in this study that LPC inhibits superoxide generation in fMLP- and PMA-stimulated neutrophils without affecting fMLP-induced Ca2+ mobilization and cell viability. This effect was observed with LPC dissolved in ethanol, but not with LPC stock solutions prepared in water or in BSA-containing aqueous solution with sonication. Under the same experimental conditions, platelet-activating factor primed neutrophils for superoxide generation. The inhibitory effect of LPC was observed within 30 s after its application and was maximal at LPC concentrations between 0.1 and 1 μM. Inhibition of superoxide generation was accompanied by a 2.5-fold increase in the intracellular cAMP concentration. In addition, LPC reduced fMLP-stimulated phosphorylation of ERK and Akt and membrane translocation of p67phox and p47phox. The protein kinase A inhibitors H-89 and adenosine 3′5′-cyclic monophosphorothioate Rp-isomer (Rp-cAMP) partially restored superoxide production in LPC-treated neutrophils, indicating involvement of protein kinase A in LPC-mediated inhibition. Using an ex vivo mouse lung perfusion model that measures lung weight change and capillary filtration coefficient, we found that LPC prevented lung vascular injury mediated by fMLP-activated neutrophils. Taken together, these results suggest that LPC-induced elevation of intracellular cAMP is partially responsible for its inhibition of neutrophil NADPH oxidase activation. A similar mechanism of inhibition may be used for the control of neutrophil-mediated tissue injury.

Original languageEnglish (US)
Pages (from-to)2981-2989
Number of pages9
JournalJournal of Immunology
Volume174
Issue number5
StatePublished - Mar 1 2005
Externally publishedYes

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Lysophosphatidylcholines
Oxidants
Cyclic AMP
Neutrophils
Superoxides
Cyclic AMP-Dependent Protein Kinases
Lung
Sonication
NADPH Oxidase
Platelet Activating Factor
Vascular System Injuries
Lung Injury
Protein Kinase Inhibitors
Adenosine
Phospholipids
Cell Survival
Ethanol
Perfusion

ASJC Scopus subject areas

  • Immunology

Cite this

Lin, P., Welch, E. J., Gao, X. P., Malik, A. B., & Ye, R. D. (2005). Lysophosphatidylcholine modulates neutrophil oxidant production through elevation of cyclic AMP. Journal of Immunology, 174(5), 2981-2989.

Lysophosphatidylcholine modulates neutrophil oxidant production through elevation of cyclic AMP. / Lin, Phoebe; Welch, Emily J.; Gao, Xiao Pei; Malik, Asrar B.; Ye, Richard D.

In: Journal of Immunology, Vol. 174, No. 5, 01.03.2005, p. 2981-2989.

Research output: Contribution to journalArticle

Lin, P, Welch, EJ, Gao, XP, Malik, AB & Ye, RD 2005, 'Lysophosphatidylcholine modulates neutrophil oxidant production through elevation of cyclic AMP', Journal of Immunology, vol. 174, no. 5, pp. 2981-2989.
Lin, Phoebe ; Welch, Emily J. ; Gao, Xiao Pei ; Malik, Asrar B. ; Ye, Richard D. / Lysophosphatidylcholine modulates neutrophil oxidant production through elevation of cyclic AMP. In: Journal of Immunology. 2005 ; Vol. 174, No. 5. pp. 2981-2989.
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