Luteinizing hormone β-subunit mRNA amounts increase during the preovulatory surge of luteinizing hormone in the ewe: The highest levels are observed at the completion of the peak

T. Landefeld, Richard Maurer, J. Kepa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Amounts of mRNA for luteinizing hormone (LH) β-subunit were assessed during selected times in the normal estrous cycle of sheep. These times spanned the luteal phase through the preovulatory LH surge, and included groups designated as: (i) day 12 of the cycle (day 12); (ii) 24 hr before the onset of the expected behavioral estrus (E - 24); (iii) 5, 15, and 25 hr after the onset of behavioral estrus (E + 5, E + 15 and E + 25). These groups were characterized by criteria such as serum and pituitary LH concentrations, serum progesterone, and ovarian morphology. Specific LH β-subunit mRNA was evaluated by Northern transfers, using a specific bovine LH β cDNA probe. Slopes were determined using a linear regression analysis and then expressed as fold stimulation relative to the values for the day 12 group. The results indicate that LH β mRNA amounts increase after day 12, reaching a value of ~12-fold by E + 25. When correlated with serum and pituitary LH amounts, it is observed that the amounts of β mRNA increase in a fashion parallel to serum and pituitary LH concentrations through the time E + 5. After this time, LH β mRNA amounts continue to increase despite a dramatic fall in both serum and pituitary LH. These results suggest that LH β mRNA amounts are regulated during the LH preovulatory surge.

Original languageEnglish (US)
Pages (from-to)249-254
Number of pages6
JournalDNA
Volume4
Issue number3
StatePublished - 1985
Externally publishedYes

Fingerprint

Luteinizing Hormone
Messenger RNA
Pituitary Hormones
Serum
Estrus
Estrous Cycle
Luteal Phase
Linear regression
Regression analysis
Progesterone
Linear Models
Sheep
Complementary DNA
Regression Analysis

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

@article{38a68b0f46ce4615b3809602f7cbe9cc,
title = "Luteinizing hormone β-subunit mRNA amounts increase during the preovulatory surge of luteinizing hormone in the ewe: The highest levels are observed at the completion of the peak",
abstract = "Amounts of mRNA for luteinizing hormone (LH) β-subunit were assessed during selected times in the normal estrous cycle of sheep. These times spanned the luteal phase through the preovulatory LH surge, and included groups designated as: (i) day 12 of the cycle (day 12); (ii) 24 hr before the onset of the expected behavioral estrus (E - 24); (iii) 5, 15, and 25 hr after the onset of behavioral estrus (E + 5, E + 15 and E + 25). These groups were characterized by criteria such as serum and pituitary LH concentrations, serum progesterone, and ovarian morphology. Specific LH β-subunit mRNA was evaluated by Northern transfers, using a specific bovine LH β cDNA probe. Slopes were determined using a linear regression analysis and then expressed as fold stimulation relative to the values for the day 12 group. The results indicate that LH β mRNA amounts increase after day 12, reaching a value of ~12-fold by E + 25. When correlated with serum and pituitary LH amounts, it is observed that the amounts of β mRNA increase in a fashion parallel to serum and pituitary LH concentrations through the time E + 5. After this time, LH β mRNA amounts continue to increase despite a dramatic fall in both serum and pituitary LH. These results suggest that LH β mRNA amounts are regulated during the LH preovulatory surge.",
author = "T. Landefeld and Richard Maurer and J. Kepa",
year = "1985",
language = "English (US)",
volume = "4",
pages = "249--254",
journal = "DNA and Cell Biology",
issn = "1044-5498",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Luteinizing hormone β-subunit mRNA amounts increase during the preovulatory surge of luteinizing hormone in the ewe

T2 - The highest levels are observed at the completion of the peak

AU - Landefeld, T.

AU - Maurer, Richard

AU - Kepa, J.

PY - 1985

Y1 - 1985

N2 - Amounts of mRNA for luteinizing hormone (LH) β-subunit were assessed during selected times in the normal estrous cycle of sheep. These times spanned the luteal phase through the preovulatory LH surge, and included groups designated as: (i) day 12 of the cycle (day 12); (ii) 24 hr before the onset of the expected behavioral estrus (E - 24); (iii) 5, 15, and 25 hr after the onset of behavioral estrus (E + 5, E + 15 and E + 25). These groups were characterized by criteria such as serum and pituitary LH concentrations, serum progesterone, and ovarian morphology. Specific LH β-subunit mRNA was evaluated by Northern transfers, using a specific bovine LH β cDNA probe. Slopes were determined using a linear regression analysis and then expressed as fold stimulation relative to the values for the day 12 group. The results indicate that LH β mRNA amounts increase after day 12, reaching a value of ~12-fold by E + 25. When correlated with serum and pituitary LH amounts, it is observed that the amounts of β mRNA increase in a fashion parallel to serum and pituitary LH concentrations through the time E + 5. After this time, LH β mRNA amounts continue to increase despite a dramatic fall in both serum and pituitary LH. These results suggest that LH β mRNA amounts are regulated during the LH preovulatory surge.

AB - Amounts of mRNA for luteinizing hormone (LH) β-subunit were assessed during selected times in the normal estrous cycle of sheep. These times spanned the luteal phase through the preovulatory LH surge, and included groups designated as: (i) day 12 of the cycle (day 12); (ii) 24 hr before the onset of the expected behavioral estrus (E - 24); (iii) 5, 15, and 25 hr after the onset of behavioral estrus (E + 5, E + 15 and E + 25). These groups were characterized by criteria such as serum and pituitary LH concentrations, serum progesterone, and ovarian morphology. Specific LH β-subunit mRNA was evaluated by Northern transfers, using a specific bovine LH β cDNA probe. Slopes were determined using a linear regression analysis and then expressed as fold stimulation relative to the values for the day 12 group. The results indicate that LH β mRNA amounts increase after day 12, reaching a value of ~12-fold by E + 25. When correlated with serum and pituitary LH amounts, it is observed that the amounts of β mRNA increase in a fashion parallel to serum and pituitary LH concentrations through the time E + 5. After this time, LH β mRNA amounts continue to increase despite a dramatic fall in both serum and pituitary LH. These results suggest that LH β mRNA amounts are regulated during the LH preovulatory surge.

UR - http://www.scopus.com/inward/record.url?scp=0021824247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021824247&partnerID=8YFLogxK

M3 - Article

C2 - 4040005

AN - SCOPUS:0021824247

VL - 4

SP - 249

EP - 254

JO - DNA and Cell Biology

JF - DNA and Cell Biology

SN - 1044-5498

IS - 3

ER -