Lunate arthroplasty with autologous mesenchymal stem cells in a rabbit model

Jerry I. Huang, Mahidhar M. Durbhakula, Peter Angele, Brian Johnstone, Jung Yoo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: There is no ideal treatment for end-stage degenerative wrist disorders and subsequent carpal collapse. The purpose of this study was to investigate whether autologous cartilage constructs tissue-engineered from bone-marrow-derived mesenchymal stem cells can be effective for carpal bone reconstruction. Methods: Total lunate excision was performed in twenty-seven adult New Zealand White rabbits. Mesenchymal stem cells were isolated from marrow and then were culture-expanded. Group-1 rabbits underwent excision only. Group-2 animals underwent excision followed by implantation of a scaffold consisting of gelatin and hyaluronan. Group-3 animals underwent excision followed by implantation of a mesenchymal stem cell-seeded scaffold that had been preincubated in chondrogenic medium. The group-1 animals were killed at six weeks, whereas the group-2 and group-3 animals were killed at six or twelve weeks. Tissues were harvested for radiographic and histologic analysis. Results: Significant carpal collapse (a 5.4% ± 2.8% reduction in the carpometacarpal index, p <0.05) was observed in the group-1 animals by six weeks. In contrast, the carpal height was maintained in the group-2 and 3 animals. There was no radiographic evidence of ossification in the group-1 or 2 animals, whereas there was radiographic evidence of ossification in all six group-3 rabbits killed at the twelve-week time-point. Histologic sections from the group-3 animals showed filling of the lunate space with islands of cartilage with interspersed bone ossicles at six weeks. At twelve weeks, there was abundant bone formation as well as evidence of neovascularization. Osseous tissue was present in the central portions of the constructs while the periphery was lined with cartilage. In groups 1 and 2, the lunate space was filled with poorly organized fibrous tissue. Conclusions: Cartilaginous implants preformed from autologous mesenchymal stem cells seeded onto biodegradable scaffold can prevent carpal collapse. The newly formed osteochondral tissue appears to function as an adequate biologic lunate spacer for at least twelve weeks in this animal model. Clinical Relevance: To our knowledge, this is the first report of whole-bone reconstruction performed with the use of mesenchymal stem cells. Biologic constructs that are tissue-engineered from mesenchymal stem cells may be a new alternative for carpal arthroplasty in patients with clinical conditions such as osteonecrosis.

Original languageEnglish (US)
Pages (from-to)744-752
Number of pages9
JournalJournal of Bone and Joint Surgery - Series A
Volume88
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

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Mesenchymal Stromal Cells
Arthroplasty
Wrist
Rabbits
Osteogenesis
Cartilage
Bone Marrow
Carpal Bones
Bone and Bones
Osteonecrosis
Hyaluronic Acid
Gelatin
Animal Models

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Lunate arthroplasty with autologous mesenchymal stem cells in a rabbit model. / Huang, Jerry I.; Durbhakula, Mahidhar M.; Angele, Peter; Johnstone, Brian; Yoo, Jung.

In: Journal of Bone and Joint Surgery - Series A, Vol. 88, No. 4, 04.2006, p. 744-752.

Research output: Contribution to journalArticle

Huang, Jerry I. ; Durbhakula, Mahidhar M. ; Angele, Peter ; Johnstone, Brian ; Yoo, Jung. / Lunate arthroplasty with autologous mesenchymal stem cells in a rabbit model. In: Journal of Bone and Joint Surgery - Series A. 2006 ; Vol. 88, No. 4. pp. 744-752.
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abstract = "Background: There is no ideal treatment for end-stage degenerative wrist disorders and subsequent carpal collapse. The purpose of this study was to investigate whether autologous cartilage constructs tissue-engineered from bone-marrow-derived mesenchymal stem cells can be effective for carpal bone reconstruction. Methods: Total lunate excision was performed in twenty-seven adult New Zealand White rabbits. Mesenchymal stem cells were isolated from marrow and then were culture-expanded. Group-1 rabbits underwent excision only. Group-2 animals underwent excision followed by implantation of a scaffold consisting of gelatin and hyaluronan. Group-3 animals underwent excision followed by implantation of a mesenchymal stem cell-seeded scaffold that had been preincubated in chondrogenic medium. The group-1 animals were killed at six weeks, whereas the group-2 and group-3 animals were killed at six or twelve weeks. Tissues were harvested for radiographic and histologic analysis. Results: Significant carpal collapse (a 5.4{\%} ± 2.8{\%} reduction in the carpometacarpal index, p <0.05) was observed in the group-1 animals by six weeks. In contrast, the carpal height was maintained in the group-2 and 3 animals. There was no radiographic evidence of ossification in the group-1 or 2 animals, whereas there was radiographic evidence of ossification in all six group-3 rabbits killed at the twelve-week time-point. Histologic sections from the group-3 animals showed filling of the lunate space with islands of cartilage with interspersed bone ossicles at six weeks. At twelve weeks, there was abundant bone formation as well as evidence of neovascularization. Osseous tissue was present in the central portions of the constructs while the periphery was lined with cartilage. In groups 1 and 2, the lunate space was filled with poorly organized fibrous tissue. Conclusions: Cartilaginous implants preformed from autologous mesenchymal stem cells seeded onto biodegradable scaffold can prevent carpal collapse. The newly formed osteochondral tissue appears to function as an adequate biologic lunate spacer for at least twelve weeks in this animal model. Clinical Relevance: To our knowledge, this is the first report of whole-bone reconstruction performed with the use of mesenchymal stem cells. Biologic constructs that are tissue-engineered from mesenchymal stem cells may be a new alternative for carpal arthroplasty in patients with clinical conditions such as osteonecrosis.",
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N2 - Background: There is no ideal treatment for end-stage degenerative wrist disorders and subsequent carpal collapse. The purpose of this study was to investigate whether autologous cartilage constructs tissue-engineered from bone-marrow-derived mesenchymal stem cells can be effective for carpal bone reconstruction. Methods: Total lunate excision was performed in twenty-seven adult New Zealand White rabbits. Mesenchymal stem cells were isolated from marrow and then were culture-expanded. Group-1 rabbits underwent excision only. Group-2 animals underwent excision followed by implantation of a scaffold consisting of gelatin and hyaluronan. Group-3 animals underwent excision followed by implantation of a mesenchymal stem cell-seeded scaffold that had been preincubated in chondrogenic medium. The group-1 animals were killed at six weeks, whereas the group-2 and group-3 animals were killed at six or twelve weeks. Tissues were harvested for radiographic and histologic analysis. Results: Significant carpal collapse (a 5.4% ± 2.8% reduction in the carpometacarpal index, p <0.05) was observed in the group-1 animals by six weeks. In contrast, the carpal height was maintained in the group-2 and 3 animals. There was no radiographic evidence of ossification in the group-1 or 2 animals, whereas there was radiographic evidence of ossification in all six group-3 rabbits killed at the twelve-week time-point. Histologic sections from the group-3 animals showed filling of the lunate space with islands of cartilage with interspersed bone ossicles at six weeks. At twelve weeks, there was abundant bone formation as well as evidence of neovascularization. Osseous tissue was present in the central portions of the constructs while the periphery was lined with cartilage. In groups 1 and 2, the lunate space was filled with poorly organized fibrous tissue. Conclusions: Cartilaginous implants preformed from autologous mesenchymal stem cells seeded onto biodegradable scaffold can prevent carpal collapse. The newly formed osteochondral tissue appears to function as an adequate biologic lunate spacer for at least twelve weeks in this animal model. Clinical Relevance: To our knowledge, this is the first report of whole-bone reconstruction performed with the use of mesenchymal stem cells. Biologic constructs that are tissue-engineered from mesenchymal stem cells may be a new alternative for carpal arthroplasty in patients with clinical conditions such as osteonecrosis.

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