Luminal plasma treatment for small diameter polyvinyl alcohol tubular scaffolds

Grace Pohan, Pascale Chevallier, Deirdre E.J. Anderson, John W. Tse, Yuan Yao, Matthew W. Hagen, Diego Mantovani, Monica Hinds, Evelyn K.F. Yim

Research output: Contribution to journalArticle

Abstract

Plasma-based surface modification is recognized as an effective way to activate biomaterial surfaces, and modulate their interactions with cells, extracellular matrix proteins, and other materials. However, treatment of a luminal surface of a tubular scaffold remains non-trivial to perform in small diameter tubes. Polyvinyl alcohol (PVA) hydrogel, which has been widely used for medical applications, lacks functional groups to mediate cell attachment. This poses an issue for vascular applications, as endothelialization in a vascular graft lumen is crucial to maintain long term graft patency. In this study, a Radio Frequency Glow Discharges (RFGD) treatment in the presence of NH3 was used to modify the luminal surface of 3-mm diameter dehydrated PVA vascular grafts. The grafted nitrogen containing functional groups demonstrated stability, and in vitro endothelialization was successfully maintained for at least 30 days. The plasma-modified PVA displayed a higher percentage of carbonyl groups over the untreated PVA control. Plasma treatment on PVA patterned with microtopographies was also studied, with only the concave microlenses topography demonstrating a significant increase in platelet adhesion. Thus, the study has shown the possibility of modifying a small diameter hydrogel tubular scaffold with the RFGD plasma treatment technique and demonstrated stability in ambient storage conditions for up to 30 days.

Original languageEnglish (US)
Article number117
JournalFrontiers in Bioengineering and Biotechnology
Volume7
Issue numberMAY
DOIs
StatePublished - Jan 1 2019

Fingerprint

Polyvinyl Alcohol
Polyvinyl alcohols
Scaffolds
Grafts
Blood Vessels
Plasmas
Glow discharges
Radio
Transplants
Functional groups
Hydrogels
Microlenses
Extracellular Matrix Proteins
Hydrogel
Biocompatible Materials
Medical applications
Platelets
Cell Communication
Topography
Surface treatment

Keywords

  • Ammonia
  • Endothelialization
  • Hydrogel
  • Radio-frequency
  • Stability

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

Cite this

Pohan, G., Chevallier, P., Anderson, D. E. J., Tse, J. W., Yao, Y., Hagen, M. W., ... Yim, E. K. F. (2019). Luminal plasma treatment for small diameter polyvinyl alcohol tubular scaffolds. Frontiers in Bioengineering and Biotechnology, 7(MAY), [117]. https://doi.org/10.3389/fbioe.2019.117

Luminal plasma treatment for small diameter polyvinyl alcohol tubular scaffolds. / Pohan, Grace; Chevallier, Pascale; Anderson, Deirdre E.J.; Tse, John W.; Yao, Yuan; Hagen, Matthew W.; Mantovani, Diego; Hinds, Monica; Yim, Evelyn K.F.

In: Frontiers in Bioengineering and Biotechnology, Vol. 7, No. MAY, 117, 01.01.2019.

Research output: Contribution to journalArticle

Pohan, G, Chevallier, P, Anderson, DEJ, Tse, JW, Yao, Y, Hagen, MW, Mantovani, D, Hinds, M & Yim, EKF 2019, 'Luminal plasma treatment for small diameter polyvinyl alcohol tubular scaffolds', Frontiers in Bioengineering and Biotechnology, vol. 7, no. MAY, 117. https://doi.org/10.3389/fbioe.2019.117
Pohan, Grace ; Chevallier, Pascale ; Anderson, Deirdre E.J. ; Tse, John W. ; Yao, Yuan ; Hagen, Matthew W. ; Mantovani, Diego ; Hinds, Monica ; Yim, Evelyn K.F. / Luminal plasma treatment for small diameter polyvinyl alcohol tubular scaffolds. In: Frontiers in Bioengineering and Biotechnology. 2019 ; Vol. 7, No. MAY.
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