Low Prevalence of Insulin-Like Growth Factor-I Gene Mutations in Human Growth Disorders

Rosemarie Lajara, John P. Galgani, David P. Dempsher, Dennis M. Bier, Peter Rotwein

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

In an attempt to identify genetic lesions contributing to human growth disorders, we evaluated a prospectively recruited group of children with growth failure for mutations in the insulin-like growth factor-I (IGF-I) gene. Two complementary approaches were used: Southern blot analysis to examine the large scale organization of the gene, and a solution hybridization, nuclease protection assay to identify small alterations, such as point mutations. From a total of 61 subjects studied, 52 had no organic basis for their short stature. Analysis of chromosomal DNA from these individuals failed to reveal any variation in the IGF-I gene except for a Hindlll site polymorphism which maps near the 3' end of the last IGF-I exon. No single nucleotide substitutions were found within IGF-I-coding regions. Since the frequency of the length polymorphism was the same for both normal-sized and short individuals, it is unlikely to be associated with growth abnormalities. Our results suggest that there is minimal DNA sequence variability in the human IGF-I gene and that mutations in IGF-I exons are infrequent causes of growth failure.

Original languageEnglish (US)
Pages (from-to)687-692
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume70
Issue number3
DOIs
StatePublished - Mar 1990

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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