Low plasma levels of 2-hydrdxyestrone are consistent with its rapid metabolic clearance

Shinzo Kono, David Brandon, George R. Merriam, D. Lynn Loriaux, Mortimer B. Lipsett

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Published plasma levels of the catechol estrogen 2-hydroxyestrone (2-OHE1) are comparable to those of estrone and estradiol. In light of the very high (40,000 L/d) metabolic clearance rate of 2-OHEi, these concentrations imply unreasonable production rates. We therefore re-examined plasma 2-OHE1 levels using a modified radiotnnmmoassay procedure. Plasma samples are extracted with ethyl acetate and passed over a short column of LH-20 Sephadex before equilibration with an antiserum directed against a 2-hydroxyestrone-17-(O-carboxymethyl)oxime-bovine serum albumin conjugate. Plasma 2-OHE1 concentrations are indistinguishable from blank (< 15 pg/ml) in men and non-pregnant women, but rise to ~ 200 pg/ml during pregnancy. These values for 2-OHE1 levels are consistent with the rapid metabolic clearance of this catechol estrogen.

Original languageEnglish (US)
Pages (from-to)463-472
Number of pages10
JournalSteroids
Volume36
Issue number4
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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