Low level epifluorescent detection of nanoparticles and DNA on dielectrophoretic microarrays

James P. Mccanna, Avery Sonnenberg, Michael (Mike) Heller

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Common epifluorescent microscopy lacks the sensitivity to detect low levels of analytes directly in clinical samples, such as drug delivery nanoparticles or disease related DNA biomarkers. Advanced systems such as confocal microscopes may improve detection, but several factors limit their applications. This study now demonstrates that combining an epifluorescent microscope with a dielectrophoretic (DEP) microelectrode array device enables the detection of nanoparticles and DNA biomarkers at clinically relevant levels. Using DEP microarray devices, nanoparticles and DNA biomarkers are rapidly isolated and concentrated onto specific microscopic locations where they are easily detected by epifluorescent microscopy. In this study, 40nm nanoparticles were detected down to 2-3 × 103/ul levels and DNA was detected down to the 200 pg/ml level. The synergy of epifluorescent microscopy and DEP microarray devices provides a new paradigm for DNA biomarker diagnostics and the monitoring of drug delivery nanoparticle concentrations.

Original languageEnglish (US)
Pages (from-to)863-873
Number of pages11
JournalJournal of Biophotonics
Volume7
Issue number11-12
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Microarrays
Nanoparticles
biomarkers
Biomarkers
DNA
deoxyribonucleic acid
nanoparticles
Microscopy
Microscopic examination
microscopy
Drug delivery
Equipment and Supplies
delivery
drugs
Microscopes
microscopes
Drug Monitoring
Microelectrodes
Monitoring
sensitivity

Keywords

  • Blood
  • Dielectrophoresis
  • DNA
  • Epifluorescent microscope
  • Microelectrode array
  • Nanoparticles

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Low level epifluorescent detection of nanoparticles and DNA on dielectrophoretic microarrays. / Mccanna, James P.; Sonnenberg, Avery; Heller, Michael (Mike).

In: Journal of Biophotonics, Vol. 7, No. 11-12, 01.01.2014, p. 863-873.

Research output: Contribution to journalArticle

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