Low density lipoprotein receptor-related protein is expressed early and becomes restricted to a somatodendritic domain during neuronal differentiation in culture

Morry D. Brown, Gary A. Banker, Isa M. Hussaini, Steven L. Gonias, Scott R. VandenBerg

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Low density lipoprotein receptor-related protein (LRP) is a multi-functional receptor which mediates the endocytotic uptake of several ligands implicated in neuronal pathophysiology. In this study, LRP expression and localization, in cultured hippocampal neurons from 18-day-old rats, were examined by immunofluorescence microscopy. LRP was restricted to the cell bodies and dendrites of mature neurons, where it was uniformly distributed on both dendritic shafts and spines. Immunoreactive protein was detected within the first 24 h of culture and acquired a polarized distribution by the end of the first week. Expression of LRP mRNA by the cultured neurons was demonstrated by Northern blot analysis. Binding studies with the LRP ligand, activated α2-macroglobulin, confirmed that LRP was present and functional on the hippocampal neuron cell surface. These studies demonstrate that neuronal LRP undergoes selective compartmentation during neuronal maturation and suggest that LRP-mediated endocytosis is largely restricted to the somatodendritic compartment.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalBrain research
Volume747
Issue number2
DOIs
StatePublished - Feb 7 1997

Keywords

  • axon
  • dendrite
  • low density lipoprotein receptor-related protein
  • neuronal differentiation
  • α-macroglobulin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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