Low CD27 expression in plasma cell dyscrasias correlates with high-risk disease: An immunohistochemical analysis

Terry Morgan, Shuchun Zhao, Karen L. Chang, Terri L. Haddix, Elisabeth Domanay, P. Joanne Cornbleet, Daniel A. Arber, Yasodha Natkunam

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Genome-wide expression studies using complementary DNA microarrays recently suggested a number of intriguing candidate genes for distinguishing plasma cell dyscrasias. Our objective was to test select markers using immunohistochemical analysis and a tissue microarray from paraffin-embedded bone marrow core biopsy specimens obtained from 8 patients with monoclonal gammopathy of undetermined significance, 17 with plasmacytoma, 160 with multiple myeloma, and 15 with plasma cell leukemia (PCL). We immunostained serial sections for CD138, CD27, CD56, p27, Ki-67, CD3, and CD20. Each core was scored in duplicate by observers blinded to phenotype and reported as the average percentage of CD138+ cells. The Mann-Whitney U test was used to determine significance between groups. PCL showed significantly less immunostaining for CD27 (P <.01) and p27 (P <.05) compared with plasmacytoma and multiple myeloma. Low CD27 expression also was associated with plasmacytoma progression to multiple myeloma (P <.05). Our results support the hypothesis that low CD27 expression correlates with high-risk disease, including primary PCL and decreased progression-free survival in solitary plasmacytoma.

Original languageEnglish (US)
Pages (from-to)545-551
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume126
Issue number4
DOIs
StatePublished - Oct 2006

Fingerprint

Paraproteinemias
Plasmacytoma
Plasma Cell Leukemia
Multiple Myeloma
Tissue Array Analysis
Monoclonal Gammopathy of Undetermined Significance
Nonparametric Statistics
Oligonucleotide Array Sequence Analysis
Paraffin
Disease-Free Survival
Complementary DNA
Bone Marrow
Genome
Phenotype
Biopsy
Genes

Keywords

  • CD27
  • MGUS
  • Monoclonal gammopathy of undetermined significance
  • Myeloma
  • Plasma cell leukemia
  • Plasmacytoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Low CD27 expression in plasma cell dyscrasias correlates with high-risk disease : An immunohistochemical analysis. / Morgan, Terry; Zhao, Shuchun; Chang, Karen L.; Haddix, Terri L.; Domanay, Elisabeth; Cornbleet, P. Joanne; Arber, Daniel A.; Natkunam, Yasodha.

In: American Journal of Clinical Pathology, Vol. 126, No. 4, 10.2006, p. 545-551.

Research output: Contribution to journalArticle

Morgan, Terry ; Zhao, Shuchun ; Chang, Karen L. ; Haddix, Terri L. ; Domanay, Elisabeth ; Cornbleet, P. Joanne ; Arber, Daniel A. ; Natkunam, Yasodha. / Low CD27 expression in plasma cell dyscrasias correlates with high-risk disease : An immunohistochemical analysis. In: American Journal of Clinical Pathology. 2006 ; Vol. 126, No. 4. pp. 545-551.
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